Butiulca Mihaela, Farczadi Lenard, Vari Camil Eugen, Imre Silvia, Azamfirei Leonard, Lazar Alexandra
Department of Anesthesiology and Intensive Care Medicine, Faculty of General Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Târgu Mureș, Romania.
Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Târgu Mureș, Romania.
Int J Mol Sci. 2024 Dec 16;25(24):13487. doi: 10.3390/ijms252413487.
Ropivacaine, a widely used regional anesthetic also used for pain management, has been increasingly used in recent years due to its increased efficacy and improved safety compared to similar anesthetics. Biomonitoring of ropivacaine and its metabolites during and after anesthesia is an essential process for ensuring therapeutic efficacy and safe usage for patients. The most useful biomonitoring tool in recent years has been liquid chromatography coupled with mass spectrometry (LC-MS/MS), which offers selectivity, sensitivity, as well as accuracy of measurements. The current manuscript summarizes and discusses the existing liquid chromatographic methods described in the literature, as well as the personal experience with developing bioanalytical and analytical methods for the quantification of ropivacaine in biological samples for clinical applications. It is focused on methodological aspects, recent advancements, challenges, and future perspectives, highlighting the importance of LC-MS/MS techniques in ropivacaine analysis.
罗哌卡因是一种广泛使用的局部麻醉剂，也用于疼痛管理，近年来因其与同类麻醉剂相比疗效增强且安全性提高而越来越多地被使用。在麻醉期间和麻醉后对罗哌卡因及其代谢物进行生物监测是确保患者治疗效果和安全用药的重要过程。近年来最有用的生物监测工具是液相色谱-质谱联用（LC-MS/MS），它具有选择性、灵敏度以及测量准确性。本手稿总结并讨论了文献中描述的现有液相色谱方法，以及开发用于临床应用的生物样品中罗哌卡因定量分析的生物分析和分析方法的个人经验。它侧重于方法学方面、最新进展、挑战和未来展望，强调了LC-MS/MS技术在罗哌卡因分析中的重要性。
