Molecular Neurobiology Laboratory, Medical Biotechnology Center, Department of Molecular Medicine, University of Southern Denmark, J.B. Winslows Vej 25, DK-5000 Odense C, Denmark.
Cereb Cortex. 2010 Aug;20(8):1904-14. doi: 10.1093/cercor/bhp261. Epub 2009 Dec 2.
Expression of the transcriptional repressor Zbtb20 is confined to the hippocampal primordium of the developing dorsal midline cortex in mice. Here, we show that misexpression of Zbtb20 converts projection neurons of the subiculum and postsubiculum (dorsal presubiculum) to CA1 pyramidal neurons that are innervated by Schaffer collateral projections in ectopic strata oriens and radiatum. The Zbtb20-transformed neurons express Bcl11B, Satb2, and Calbindin-D28k, which are markers of adult CA1 pyramidal neurons. Downregulation of Zbtb20 expression by RNA interference impairs the normal maturation of CA1 pyramidal neurons resulting in deficiencies in Calbindin-D28k expression and in reduced apical dendritic arborizations in stratum lacunosum moleculare. Overall, the results show that Zbtb20 is required for various aspects of CA1 pyramidal neuron development such as the postnatal extension of apical dendritic arbors in the distal target zone and the subtype differentiation of Calbindin-D28k-positive subsets. They further suggest that Zbtb20 plays a role in arealization of the midline cortex.
转录抑制因子 Zbtb20 的表达局限于发育中的背侧中线皮层海马原基。在这里，我们表明 Zbtb20 的异位表达将下托和下托后的投射神经元（背侧前下托）转化为 CA1 锥体神经元，这些神经元被 Schaffer 侧支投射支配在异位的层或辐射层。Zbtb20 转化的神经元表达 Bcl11B、Satb2 和 Calbindin-D28k，这是成年 CA1 锥体神经元的标志物。RNA 干扰下调 Zbtb20 的表达会损害 CA1 锥体神经元的正常成熟，导致 Calbindin-D28k 表达减少和在分子层的腔隙状突中的树突分支减少。总的来说，这些结果表明 Zbtb20 对于 CA1 锥体神经元发育的各个方面都是必需的，例如在远侧靶区的树突分支的出生后延伸和 Calbindin-D28k 阳性亚群的亚型分化。它们进一步表明 Zbtb20 在中线皮层的区域化中发挥作用。
