通过全内反射全息显微镜对细胞黏附进行定量成像。

Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy.

作者信息

Ash William M, Krzewina Leo, Kim Myung K

机构信息

Digital Holography and Microscopy Laboratory, Department of Physics, University of South Florida, Tampa, Florida 33620, USA.

出版信息

Appl Opt. 2009 Dec 1;48(34):H144-52. doi: 10.1364/AO.48.00H144.

DOI:10.1364/AO.48.00H144

PMID:19956284

Abstract

Total internal reflection (TIR) holographic microscopy uses a prism in TIR as a near-field imager to perform quantitative phase microscopy of cell-substrate interfaces. The presence of microscopic organisms, cell-substrate interfaces, adhesions, and tissue structures on the prism's TIR face causes relative index of refraction and frustrated TIR to modulate the object beam's evanescent wave phase front. We present quantitative phase images of test specimens such as Amoeba proteus and cells such as SKOV-3 and 3T3 fibroblasts.

摘要

全内反射（TIR）全息显微镜使用处于全内反射状态的棱镜作为近场成像仪，对细胞-基质界面进行定量相显微镜观察。棱镜全内反射面上存在的微生物、细胞-基质界面、黏附物和组织结构会导致相对折射率和受阻全内反射来调制物光束的倏逝波相位前沿。我们展示了诸如大变形虫等测试样本以及SKOV-3和3T3成纤维细胞等细胞的定量相图像。