de Silva Suresh, Bowers William J
Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, USA;
Viruses. 2009 Dec 1;1(3):594-629. doi: 10.3390/v1030594.
Since its emergence onto the gene therapy scene nearly 25 years ago, the replication-defective Herpes Simplex Virus Type-1 (HSV-1) amplicon has gained significance as a versatile gene transfer platform due to its extensive transgene capacity, widespread cellular tropism, minimal immunogenicity, and its amenability to genetic manipulation. Herein, we detail the recent advances made with respect to the design of the HSV amplicon, its numerous in vitro and in vivo applications, and the current impediments this virus-based gene transfer platform faces as it navigates a challenging path towards future clinical testing.
自近25年前出现在基因治疗领域以来，复制缺陷型单纯疱疹病毒1型（HSV-1）扩增子因其广泛的转基因能力、广泛的细胞嗜性、最小的免疫原性以及易于基因操作，已成为一种多功能的基因转移平台。在此，我们详细介绍了HSV扩增子设计方面的最新进展、其众多的体外和体内应用，以及这个基于病毒的基因转移平台在迈向未来临床试验的艰难道路上面临的当前障碍。
