Department of Surgery, Key Laboratory of Carcinogenesis and Translational Research, Peking University School of Oncology, Beijing Cancer Hospital and Institute, Beijing, P.R. China.
Oncol Rep. 2010 Jan;23(1):45-52.
To investigate whether S100A14 and S100A4 expression correlates with metastatic potential and prognosis in colorectal cancer (CRC), we firstly used RT-PCR analysis to detect mRNA expression of S100A14 and S100A4 in 40 pairs of fresh tumor samples matched with adjacent normal tissues. We then evaluated the clinical significance of our findings with immunohistochemistry on 115 samples of formalin-fixed and paraffin-embedded tumors on tissue microarrays. Typically, we identified decreased S100A14 mRNA levels (52.5%, 21/40), and increased S100A4 mRNA levels (70.0%, 28/40) in primary CRC samples. In addition, down-regulated or absent S100A14 expression was detected in 56.5% of samples (65/115) and was correlated with poor differentiation (P=0.010). In contrast, overexpressed S100A4 was detected in 57.4% of samples (66/115) and was associated with lymph node metastasis (P=0.001). Simultaneous S100A14 low-expression and S100A4 high-expression was correlated with high CRC metastatic potential (P<0.001). Taken together, the signature derived from the combined expression status of S100A14 and S100A4 could be a valuable prognostic indicator in CRC.
为了研究 S100A14 和 S100A4 的表达是否与结直肠癌（CRC）的转移潜能和预后相关，我们首先使用 RT-PCR 分析检测了 40 对新鲜肿瘤样本及其配对的相邻正常组织中 S100A14 和 S100A4 的 mRNA 表达。然后，我们使用组织微阵列上的 115 例福尔马林固定和石蜡包埋肿瘤的免疫组织化学评估了我们发现的临床意义。通常，我们在原发性 CRC 样本中鉴定出 S100A14 mRNA 水平降低（52.5%，21/40）和 S100A4 mRNA 水平升高（70.0%，28/40）。此外，在 56.5%的样本（65/115）中检测到下调或缺失的 S100A14 表达，并且与低分化相关（P=0.010）。相比之下，在 57.4%的样本（66/115）中检测到 S100A4 的过表达，并且与淋巴结转移相关（P=0.001）。同时，S100A14 低表达和 S100A4 高表达与 CRC 高转移潜能相关（P<0.001）。总之，源自 S100A14 和 S100A4 联合表达状态的特征可能是 CRC 中一种有价值的预后指标。
