School of Biotechnology, Aging-associated Vascular Disease Research Center, Yeungnam University, Gyeongsan, 712-749, Korea.
Int J Mol Med. 2010 Jan;25(1):129-36.
To compare the molecular composition and functional differences at the lipoprotein level, we analyzed individual lipoprotein fractions from male patients with metabolic syndrome (MetS) (n=10) and gender- and age-matched healthy controls (n=14). The MetS group had significantly higher obesity, blood pressure, serum cholesterol, triglyceride (TG), adiponectin, and uric acid levels than the control group, while the serum blood urea nitrogen and creatinine levels of the MetS group were in the normal range. The MetS group had much weaker serum antioxidant ability and were more susceptible to copper-mediated low-density lipoprotein (LDL)-oxidation. TG and apoC-III co-accumulated with LDL, high density lipoprotein (HDL)2, and HDL3 in the MetS group. The MetS group had serum amyloid A (SAA)-enriched HDL2 and HDL3, although the serum level of SAA was not higher than in controls. The MetS group had significantly deprived paraoxonase (PON) activity in the serum and HDL, while the MetS group had 38% higher serum cholesteryl ester transfer protein (CETP) activity than that of the control group. Many serum parameters, such as TG, apoC-III, and uric acid, were elevated in the MetS group, and most of these measures were enriched in the LDL and HDL fractions rather than the very low density lipoprotein (VLDL) fraction. The lipid and apolipoprotein composition of HDL was severely altered and its beneficial functions were severely diminished. ApoA-I level was more readily detected in lipoprotein-deficient serum of the MetS group, indicating that the apoA-I exists in a lipid-free state. These results suggest that the MetS group had dysfunctional HDL that enriched TG, apoC-III, CETP, and SAA without antioxidant activity.
为了比较脂蛋白水平上的分子组成和功能差异，我们分析了患有代谢综合征（MetS）的男性患者（n=10）和性别及年龄匹配的健康对照组（n=14）的个体脂蛋白分数。MetS 组的肥胖、血压、血清胆固醇、甘油三酯（TG）、脂联素和尿酸水平明显高于对照组，而 MetS 组的血清血尿素氮和肌酐水平在正常范围内。MetS 组的血清抗氧化能力较弱，更容易发生铜介导的低密度脂蛋白（LDL）氧化。TG 和载脂蛋白 C-III 与 LDL、高密度脂蛋白（HDL）2 和 HDL3 在 MetS 组中共同积累。MetS 组的血清淀粉样蛋白 A（SAA）富含 HDL2 和 HDL3，尽管血清 SAA 水平并未高于对照组。MetS 组的血清和高密度脂蛋白中的对氧磷酶（PON）活性明显降低，而 MetS 组的血清胆固醇酯转移蛋白（CETP）活性比对照组高 38%。MetS 组的许多血清参数，如 TG、apoC-III 和尿酸，均升高，其中大多数这些指标在 LDL 和 HDL 分数中富集，而不是在极低密度脂蛋白（VLDL）分数中富集。HDL 的脂质和载脂蛋白组成严重改变，其有益功能严重降低。MetS 组的脂蛋白缺乏血清中更容易检测到载脂蛋白 A-I 水平，表明载脂蛋白 A-I 以无脂质状态存在。这些结果表明，MetS 组的 HDL 功能失调，富含 TG、apoC-III、CETP 和 SAA，而没有抗氧化活性。
