血清对氧磷酶-1 活性降低与代谢综合征患者血清淀粉样蛋白 A 水平升高有关。

Lower serum paraoxonase-1 activity is related to higher serum amyloid a levels in metabolic syndrome.

机构信息

Department of Endocrinology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

出版信息

Arch Med Res. 2011 Apr;42(3):219-25. doi: 10.1016/j.arcmed.2011.05.002.

DOI:10.1016/j.arcmed.2011.05.002

PMID:21722818

Abstract

BACKGROUND AND AIMS

High-density lipoproteins (HDL) contain the anti-oxidative enzyme, paraoxonase-1 (PON-1), which is important for atheroprotection. The acute phase reactant, serum amyloid A (SAA), an HDL-associated apolipoprotein, may impair PON-1 activity, whereas SAA and PON-1 are reciprocally regulated in response to acute inflammatory stimuli. The relationship of serum PON-1 activity with SAA during low-grade chronic inflammation is unclear. Here we tested the extent to which low serum PON-1 activity is related to high SAA levels in subjects with and without metabolic syndrome (MetS).

METHODS

In 19 nondiabetic subjects with MetS and 67 subjects without MetS, serum PON-1, assayed as its arylesterase activity, and SAA were measured together with plasma lipids and lipoproteins, high-sensitive C-reactive protein (hs-CRP) and insulin resistance (homeostasis model assessment (HOMA(ir)).

RESULTS

PON-1 activity was decreased (p=0.023), whereas SAA levels were increased (p=0.042) in MetS subjects, coinciding with higher hs-CRP levels and HOMA(ir) values. Multiple linear regression analysis revealed that age- and gender-adjusted PON-1 activity was related inversely to SAA (β=-0.256, p=0.020) after adjustment for MetS, or alternatively for hs-CRP and HOMA(ir) (β=-0.271, p=0.049).

CONCLUSIONS

Decreased serum PON-1 activity in MetS may in part be attributable to higher SAA levels. We suggest that higher SAA levels contribute to impaired HDL anti-oxidative function in MetS via an effect on PON-1 regulation.

摘要

背景与目的

高密度脂蛋白（HDL）含有抗氧化酶，对氧磷酶-1（PON-1），这对于动脉粥样硬化保护很重要。急性期反应物血清淀粉样蛋白 A（SAA）是一种与 HDL 相关的载脂蛋白，可能会损害 PON-1 活性，而 SAA 和 PON-1 则在对急性炎症刺激的反应中相互调节。在低度慢性炎症期间，血清 PON-1 活性与 SAA 的关系尚不清楚。在这里，我们检测了在有和没有代谢综合征（MetS）的受试者中，低血清 PON-1 活性与高 SAA 水平之间的关系程度。

方法

在 19 名非糖尿病 MetS 患者和 67 名非 MetS 患者中，同时测量血清 PON-1（作为其芳基酯酶活性进行测定）和 SAA，以及血浆脂质和脂蛋白、高敏 C 反应蛋白（hs-CRP）和胰岛素抵抗（稳态模型评估（HOMA(ir)）。

结果

MetS 患者的 PON-1 活性降低（p=0.023），SAA 水平升高（p=0.042），同时 hs-CRP 水平和 HOMA(ir) 值升高。多元线性回归分析表明，在调整 MetS 或 hs-CRP 和 HOMA(ir) 后，年龄和性别调整后的 PON-1 活性与 SAA 呈负相关（β=-0.256，p=0.020）。