State Key Laboratory of Water Resource and Environment (SKLWRE), Harbin Institute of Technology, Harbin 150090, China.
Appl Microbiol Biotechnol. 2010 Mar;86(1):353-8. doi: 10.1007/s00253-009-2367-z. Epub 2009 Dec 3.
Denitrifying sulfide removal (DSR) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. This investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the correlation between the composition of the microbial community and the DSR performance. In the original suspension and in 10(-2) dilution, the strains Stenotrophomonas sp., Thauera sp., and Azoarcus sp. are the heterotrophic denitrifiers and the strains Paracoccus sp. and Pseudomonas sp. are the sulfide-oxidizing denitrifers. The 10(-4) dilution is identified as the functional consortium for the present DSR system, which comprises two functional strains, Stenotrophomonas sp. strain Paracoccus sp. At 10(-6) dilution, all DSR performance was lost. The functions of the constituent cells in the DSR granules were discussed based on data obtained using the dilution-to-extinction approach.
反硝化脱硫（DSR）工艺可将工业废水中的硫化物、硝酸盐和化学需氧量分别转化为元素硫、氮气和二氧化碳。本研究采用 10(-2) 至 10(-6) 稀释度的稀释至灭绝方法，阐明微生物群落组成与 DSR 性能之间的相关性。在原始悬浮液和 10(-2) 稀释度下，变形杆菌属、陶厄氏菌属和固氮弧菌属是异养反硝化菌，假单胞菌属和副球菌属是硫化物氧化反硝化菌。10(-4) 稀释度被确定为当前 DSR 系统的功能联合体，它由两个功能菌株，即变形杆菌属和副球菌属组成。在 10(-6) 稀释度下，所有 DSR 性能都丧失了。根据稀释至灭绝方法获得的数据，讨论了 DSR 颗粒中组成细胞的功能。
