School of Biomolecular and Biomedical Science, Centre for Synthesis and Chemical Biology, Ardmore House, University College Dublin, Dublin 4, Ireland.
Appl Microbiol Biotechnol. 2010 Mar;86(1):345-51. doi: 10.1007/s00253-009-2346-4. Epub 2009 Dec 3.
The fungus Cunninghamella elegans is a useful model of human catabolism of xenobiotics. In this paper, the biotransformation of fluorinated biphenyls by C. elegans was investigated by analysis of the culture supernatants with a variety of analytical techniques. 4-Fluorobiphenyl was principally transformed to 4-fluoro-4'-hydroxybiphenyl, but other mono- and dihydroxylated compounds were detected in organic extracts by gas chromatography-mass spectrometry. Additionally, fluorinated water-soluble products were detected by (19)F NMR and were identified as sulphate and beta-glucuronide conjugates. Other fluorobiphenyls (2-fluoro-, 4,4'-difluoro- and 2,3,4,5,6-pentafluoro-biphenyl) were catabolised by C. elegans, yielding mono- and dihydroxylated products, but phase II metabolites were detected from 4,4'-difluorobiphenyl only.
多形被孢霉是一种研究异生物质人体代谢的有用模式生物。本文采用多种分析技术，分析了多形被孢霉转化氟联苯的情况。4-氟联苯主要转化为 4-氟-4'-羟基联苯，但在有机提取物中用气相色谱-质谱检测到其他单羟基和二羟基化合物。此外，（19）F NMR 检测到氟代水溶性产物，鉴定为硫酸盐和β-葡糖苷酸结合物。其他氟联苯（2-氟、4,4'-二氟和 2,3,4,5,6-五氟联苯）也被多形被孢霉代谢，生成单羟基和二羟基产物，但仅从 4,4'-二氟联苯检测到相 II 代谢产物。
