Biol Proced Online. 2009 Dec 3;11:325-30. doi: 10.1007/s12575-009-9018-8.
Analgesic and anesthetic drugs may have an impact on the results achieved from animal experiments. In the study presented here, we try to enlighten whether anesthesia with fentanyl/fluniasone and midazolam (Hypnorm and Dormicum) has an influence on measurements of hemoglobin in mice. In a cross-over study, we have compared hemoglobin levels in two groups of mice: anesthetized versus non-anesthetized and found significant decrease in hemoglobin levels in the anesthetized group (p < 0.05) unrelated to which group received the anesthesia. The mean hemoglobin levels after intraperitoneal administration of Hypnorm and Dormicum was 8.7 mmol/L compared to mean hemoglobin 9.9 mmol/L before anesthesia (p < 0.001), and the decrease lasted for more than 30 min. These results show that anesthesia can be an important confounder in studies involving measurements of hemoglobin, and this should be taken into account when planning studies and analyzing data.
镇痛和麻醉药物可能会影响动物实验的结果。在本研究中，我们试图阐明芬太尼/氟尼缩松和咪达唑仑（Hypnorm 和 Dormicum）麻醉是否会影响小鼠血红蛋白的测量。在一项交叉研究中，我们比较了两组小鼠的血红蛋白水平：麻醉组与非麻醉组，并发现麻醉组的血红蛋白水平显著下降（p<0.05），与接受哪种麻醉无关。Hypnorm 和 Dormicum 腹腔给药后的平均血红蛋白水平为 8.7mmol/L，而麻醉前的平均血红蛋白水平为 9.9mmol/L（p<0.001），且下降持续了 30 多分钟。这些结果表明，麻醉可能是涉及血红蛋白测量的研究中的一个重要混杂因素，在计划研究和分析数据时应考虑到这一点。
