Hussain M, Hastings J G, White P J
Department of Molecular Biology and Biotechnology, The University, Sheffield, United Kingdom.
J Infect Dis. 1991 Mar;163(3):534-41. doi: 10.1093/infdis/163.3.534.
Slime isolated after growth of four strains of coagulase-negative staphylococci on chemically defined medium plus agar was rich in galactose. However, when sterile agar plates were extracted with saline, high-molecular-weight material with similar properties was obtained that also was galactose-rich. Most of the dry weight attributed to slime, and probably all the galactose, originated from agar. Slime isolated by gel and ion-exchange chromatography from liquid culture in the same medium contained glycerol phosphate, glucose (no galactose), glucosamine, alanine, uronate, an unidentified component, and protein. Separation of protein from carbohydrate was achieved by affinity chromatography. [14C]glucose in the medium labeled the carbohydrate polymer; [14C]amino acids chiefly labeled extracellular proteins. Slime from bacteria grown on medium solidified with silica gel or on dialysis membrane above an agar surface showed essentially the same composition and behavior after purification as the material isolated from liquid culture.
在化学成分确定的培养基加琼脂上培养四株凝固酶阴性葡萄球菌后分离得到的黏液富含半乳糖。然而，当用盐水提取无菌琼脂平板时，得到了具有相似性质的高分子量物质，该物质同样富含半乳糖。归因于黏液的大部分干重，可能还有所有的半乳糖，都源自琼脂。在相同培养基的液体培养物中通过凝胶和离子交换色谱法分离得到的黏液含有甘油磷酸、葡萄糖（无半乳糖）、氨基葡萄糖、丙氨酸、糖醛酸、一种未鉴定成分和蛋白质。通过亲和色谱法实现了蛋白质与碳水化合物的分离。培养基中的[¹⁴C]葡萄糖标记了碳水化合物聚合物；[¹⁴C]氨基酸主要标记细胞外蛋白质。在硅胶固化的培养基上或琼脂表面上方的透析膜上生长的细菌产生的黏液，纯化后其组成和行为与从液体培养物中分离得到的物质基本相同。
