Kotilainen P, Oksman P, Viljanen M K, Nikoskelainen J, Huovinen P
Department of Medical Microbiology, University of Turku, Finland.
Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):873-9. doi: 10.1007/BF01967502.
Gas-liquid chromatography-mass spectrometry was used to analyze the extracellular extracts of 108 coagulase-negative staphylococcal strains for the presence of mannose, galactose, glucose and ribose, in order to determine whether production of these four monosaccharides, regarded as potential staphylococcal slime components, was associated with the adherence capacity of the individuals strains. A total of 90 Staphylococcus epidermidis and 18 Staphylococcus hominis strains were studied. Using the quantitative spectrophotometric assay, 21 Staphylococcus epidermidis strains were classified as strongly adherent, 12 as moderately adherent, 11 as weakly adherent, and 46 as nonadherent. All 18 Staphylococcus hominis strains were nonadherent. Mannose, galactose, glucose and ribose were detected as the main monosaccharide components in the extracellular extracts of all strains examined. Moreover, the mean relative concentrations of these monosaccharides were essentially the same for the different adherence phenotypes within the species Staphylococcus epidermidis. These results showed that there was no causal connection between the adherence of coagulase-negative staphylococci and the extracellular production of any of the four monosaccharides analyzed.
采用气-液色谱-质谱联用技术分析108株凝固酶阴性葡萄球菌菌株的细胞外提取物中甘露糖、半乳糖、葡萄糖和核糖的存在情况，以确定这四种被视为潜在葡萄球菌黏液成分的单糖的产生是否与各菌株的黏附能力相关。共研究了90株表皮葡萄球菌和18株人葡萄球菌菌株。使用定量分光光度法测定，21株表皮葡萄球菌菌株被分类为强黏附性，12株为中度黏附性，11株为弱黏附性, 46株为非黏附性。所有18株人葡萄球菌菌株均为非黏附性。在所检测的所有菌株的细胞外提取物中，甘露糖、半乳糖、葡萄糖和核糖被检测为主要的单糖成分。此外，在表皮葡萄球菌种内，这些单糖的平均相对浓度对于不同的黏附表型基本相同。这些结果表明，凝固酶阴性葡萄球菌的黏附与所分析四种单糖中任何一种的细胞外产生之间没有因果关系。
