Hussain M, Wilcox M H, White P J, Faulkner M K, Spencer R C
Department of Experimental and Clinical Microbiology, University of Sheffield.
J Hosp Infect. 1992 Mar;20(3):173-84. doi: 10.1016/0195-6701(92)90085-z.
Marked differences in both the production of slime and adherence by Staphylococcus epidermidis were observed when comparing four culture media. Slime isolated from a strain cultured in a chemically defined medium (HHW) in air was chemically indistinguishable from that formed in both HHW and synthetic dialysis fluid (SDF) in air with 5% CO2. The presence of a physiological level of CO2 during culture in tryptone soya broth (TSB) prevented production of slime. It was not possible to separate the constituents of slime from those of the culture medium in bacteria grown in TSB in air using DEAE cellulose. Slime production was notably poor in used peritoneal dialysis fluid (PUD). Adherent growth was marked in HHW and SDF but was poor in TSB and PUD when air with 5% CO2 was used. These findings emphasize the advantages in using chemically defined and biological fluids when studying slime production and adherence by S. epidermidis.
在比较四种培养基时，观察到表皮葡萄球菌在黏液产生和黏附方面存在显著差异。从在化学限定培养基（HHW）中于空气中培养的菌株分离出的黏液，与在空气中含5%二氧化碳的HHW和合成透析液（SDF）中形成的黏液在化学上无法区分。在胰蛋白胨大豆肉汤（TSB）中培养期间存在生理水平的二氧化碳会阻止黏液的产生。使用DEAE纤维素无法将在空气中TSB中生长的细菌的黏液成分与培养基成分分离。在使用过的腹膜透析液（PUD）中黏液产生明显较差。在HHW和SDF中附着生长明显，但在使用含5%二氧化碳的空气时，在TSB和PUD中附着生长较差。这些发现强调了在研究表皮葡萄球菌的黏液产生和黏附时使用化学限定和生物流体的优势。
