Department of Food Science, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil.
Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):657-63. doi: 10.1515/znc-2009-9-1009.
Phenolic compounds are found in seaweed species together with other substances presenting antioxidant activity. The objective of this work was to evaluate the antioxidant activity of the free phenolic acids (FPA) fraction from the seaweed Halimeda monile, and its activity to protect the expression of hepatic enzymes in rats, under experimental CCl4 injury. The antioxidant activity was measured by the DPPH method. The FPA fraction (80 mg/kg, p.o.) was administered during 20 consecutive days to rats. The peroxidation was performed by thiobarbituric acid reactive substances (TBARS). The SOD and CAT enzymatic expressions were measured by RT/PCR. The histology technique was used to evaluate liver injuries. The expression of both, CAT and SOD genes, was more preserved by FPA. Only partial injury could be observed by histology in the liver of rats receiving FPA as compared with the control group; and CCl4 administration induced 60% more peroxidation as compared with the rats receiving FPA. These data suggest that FPA could modulate the antioxidant enzymes and oxidative status in the liver through protection against adverse effects induced by chemical agents.
酚类化合物在海藻物种中与具有抗氧化活性的其他物质一起存在。本工作的目的是评估海藻 Halimeda monile 中游离酚酸 (FPA) 部分的抗氧化活性，以及其在实验性 CCl4 损伤下保护大鼠肝酶表达的活性。抗氧化活性通过 DPPH 法测量。FPA 部分（80mg/kg，po）连续 20 天给予大鼠。通过硫代巴比妥酸反应物质 (TBARS) 进行过氧化。通过 RT/PCR 测量 SOD 和 CAT 酶的表达。组织学技术用于评估肝损伤。与对照组相比，FPA 更能保留 CAT 和 SOD 基因的表达。与接受 FPA 的大鼠相比，用 FPA 处理的大鼠肝脏仅观察到部分损伤；并且 CCl4 给药导致过氧化增加了 60%。这些数据表明，FPA 可以通过保护化学物质诱导的不良影响来调节肝脏中的抗氧化酶和氧化状态。
