Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brasil.
Redox Rep. 2012;17(2):47-53. doi: 10.1179/1351000212Y.0000000003.
Antioxidant activity and hepatoprotective properties of the aqueous extract and tetrahydrofuran-extracted phenolic fractions of Halimeda opuntia (Linnaeus) Lamouroux were investigated in rats with chemically induced liver injury. Total polyphenols were determined by using the Folin-Ciocalteau reagent. Liver damage was induced by CCl(4) and assessed by a histological technique. Reverse transcription/polymerase chain reaction (RT/PCR) analysis showed increased superoxide dismutase (SOD) and catalase (CAT) gene expression and activities in the group treated with free phenolic acid (FPA) fractions of H. opuntia, suggesting inducing effects on both enzymes. In addition, rats treated with FPA fractions displayed lower liver thiobarbituric acid reactive substance (TBARS) levels than those observed for rats in the CCl(4)-treated group. These data suggest that the phenolic fractions from H. opuntia may protect the liver against oxidative stress-inducing effects of chemicals by modulating its antioxidant enzymes and oxidative status.
采用 Folin-Ciocalteau 试剂测定总多酚含量，研究了石莼（林奈）拉莫鲁克斯水提物和四氢呋喃提取的酚类部位对化学诱导肝损伤大鼠的抗氧化活性和肝保护作用。采用组织学技术评估肝损伤。逆转录/聚合酶链反应（RT/PCR）分析显示，用游离酚酸（FPA）处理组的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）基因表达和活性增加，表明对两种酶均有诱导作用。此外，用 FPA 处理的大鼠的肝丙二醛（TBARS）水平低于 CCl4 处理组大鼠。这些数据表明，石莼的酚类部位可能通过调节抗氧化酶和氧化状态来保护肝脏免受化学物质诱导的氧化应激。
