Institute of Biophysics, Bulgarian Academy of Sciences, Academic G. Bonchev str., bl. 21, Sofia 1113, Bulgaria.
Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):673-9. doi: 10.1515/znc-2009-9-1011.
An analysis of the shape of photosynthetic light curves is presented and the existence of the initial non-linear part is shown as a consequence of the operation of the non-cooperative (Kok's) mechanism of oxygen evolution or the effect of dark respiration. The effect of nonlinearity on the quantum efficiency (yield) and quantum requirement is reconsidered. The essential conclusions are: 1) The non-linearity of the light curves cannot be compensated using suspensions of algae or chloroplasts with high (>1.0) optical density or absorbance. 2) The values of the maxima of the quantum efficiency curves or the values of the minima of the quantum requirement curves cannot be used for estimation of the exact value of the maximum quantum efficiency and the minimum quantum requirement. The estimation of the maximum quantum efficiency or the minimum quantum requirement should be performed only after extrapolation of the linear part at higher light intensities of the quantum requirement curves to "0" light intensity.
本文分析了光合光曲线的形状，表明初始非线性部分的存在是由于非合作（Kok 的）氧气释放机制的运行或暗呼吸的影响。重新考虑了非线性对量子效率（产量）和量子需求的影响。主要结论如下：1）用光密度或吸光度大于 1.0 的藻类或叶绿体悬浮液不能补偿光曲线的非线性。2）量子效率曲线最大值或量子需求曲线最小值的值不能用于估计最大量子效率和最小量子需求的确切值。仅在量子需求曲线的较高光强度下将线性部分外推到“0”光强度后，才能对最大量子效率或最小量子需求进行估计。
