Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
Int J Mol Sci. 2023 Feb 4;24(4):3108. doi: 10.3390/ijms24043108.
The present study shows the effect of salinity on the functions of thylakoid membranes from two hybrid lines of : x and x , grown in a Hoagland solution with two NaCl concentrations (100 and 150 mM) and different exposure times (10 and 25 days). We observed inhibition of the photochemical activities of photosystem I (DCPIH → MV) and photosystem II (HO → BQ) only after the short treatment (10 days) with the higher NaCl concentration. Data also revealed alterations in the energy transfer between pigment-protein complexes (fluorescence emission ratios F/F and FF), the kinetic parameters of the oxygen-evolving reactions (initial S-S state distribution, misses (α), double hits (β) and blocked centers (S)). Moreover, the experimental results showed that after prolonged treatment with NaCl x adapted to the higher concentration of NaCl (150 mM), while this concentration is lethal for x . This study demonstrated the relationship between the salt-induced inhibition of the photochemistry of both photosystems and the salt-induced changes in the energy transfer between the pigment-protein complexes and the alterations in the Mn cluster of the oxygen-evolving complex under salt stress.
本研究表明盐度对在含有两种 NaCl 浓度（100 和 150mM）和不同暴露时间（10 和 25 天）的 Hoagland 溶液中生长的两个杂交系 x 和 x 的类囊体膜功能的影响。我们仅在较短的处理时间（10 天）内观察到较高 NaCl 浓度对光系统 I（DCPIH→MV）和光系统 II（HO→BQ）的光化学活性的抑制作用。数据还揭示了色素-蛋白复合物之间的能量转移的变化（荧光发射比 F/F 和 FF）、氧释放反应的动力学参数（初始 S-S 状态分布、缺失（α）、双击中（β）和阻塞中心（S））。此外，实验结果表明，经过长时间的 NaCl 处理，x 系适应了较高的 NaCl 浓度（150mM），而这种浓度对 x 系是致命的。本研究表明，盐度对两个光系统的光化学抑制作用之间的关系，以及盐度对色素-蛋白复合物之间的能量转移的变化以及在盐胁迫下氧释放复合物的 Mn 簇的改变之间的关系。
