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用于不同类别的核酸酶的选择性荧光化学传感器。

Selective fluorogenic chemosensors for distinct classes of nucleases.

机构信息

College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 702-701, Korea.

出版信息

Chembiochem. 2013 Mar 4;14(4):440-4. doi: 10.1002/cbic.201300001. Epub 2013 Feb 1.

DOI:10.1002/cbic.201300001
PMID:23371801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3770146/
Abstract

NUCLEASE SENSOR TRIO

Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3'-exonucleases, 5'-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.

摘要

核酸传感器三联体

针对不同类型的核酸酶（3'-核酸外切酶、5'-核酸外切酶和内切酶），开发了荧光 DNA 传感器。这些高度选择性的传感器由含有非天然荧光碱基芘的非常小的修饰 DNA 寡核苷酸构建而成，并使用胸腺嘧啶作为猝灭剂，可在多种复杂的生物介质中发挥作用。

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本文引用的文献

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In situ, real-time monitoring of the 3' to 5' exonucleases secreted by living cells.在活细胞内原位实时监测 3' 到 5' 的外切核酸酶。
Anal Chem. 2012 Jun 5;84(11):5059-65. doi: 10.1021/ac300745f. Epub 2012 May 14.
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Direct fluorescence monitoring of DNA base excision repair.DNA碱基切除修复的直接荧光监测
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Carbon nanotube-DNA hybrid used for activity monitoring and inhibitor screening of nuclease.碳纳米管-DNA 杂合体用于核酸酶的活性监测和抑制剂筛选。
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DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA.DNA 模板化整体用于无标记和实时荧光开启检测单链 DNA 的酶切/氧化切割。
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Protease probes built from DNA: multispectral fluorescent DNA-peptide conjugates as caspase chemosensors.基于DNA构建的蛋白酶探针：多光谱荧光DNA-肽缀合物作为半胱天冬酶化学传感器。
Angew Chem Int Ed Engl. 2011 May 23;50(22):5105-9. doi: 10.1002/anie.201007805. Epub 2011 Mar 31.
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Fluorescent DNA-based enzyme sensors.荧光 DNA 酶传感器。
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DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.DNA-多荧光团缔合物作为酯酶和脂肪酶的灵敏报告分子。
Chem Commun (Camb). 2010 Feb 28;46(8):1221-3. doi: 10.1039/b926338a. Epub 2010 Jan 22.
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Universal platform for sensitive and label-free nuclease assay based on conjugated polymer and DNA/intercalating dye complex.基于共轭聚合物和 DNA/嵌入染料复合物的灵敏、无标记核酸酶分析通用平台。
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Polyfluorophore labels on DNA: dramatic sequence dependence of quenching.多荧光团标记的 DNA：猝灭的显著序列依赖性。
Chemistry. 2009 Nov 2;15(43):11551-8. doi: 10.1002/chem.200901607.
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Nucleic acid-induced self-assembly of a platinum(II) terpyridyl complex: detection of G-quadruplex formation and nuclease activity.核酸诱导的铂（II）三联吡啶配合物自组装：G-四链体形成及核酸酶活性检测
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