Institute of Molecular Oncology, Biomedical Sciences Research Center "Alexander Fleming", Vari, Greece.
Crit Rev Clin Lab Sci. 2009;46(5-6):319-42. doi: 10.3109/10408360903375277.
Colorectal cancer usually develops in clearly defined stages, with distinct molecular alterations characterizing each transition. This often slow process makes colorectal cancer an ideal target for early detection programs. The blossoming of global, -omics approaches in recent years has led to greatly increased expectations for novel diagnostic and prognostic tools. Despite many early disappointments and the resulting skepticism, real progress has been made with exciting new prospects for cancer research. This review summarizes currently available proteomic tools for identifying novel biomarkers and drug targets, as well as an overview of their application in research on molecular mechanisms of carcinogenesis. Emphasis is given to novel sample preparation methods, protein separation and identification techniques, and advanced mass spectrometry tools for quantitative proteomic. The most important applications of these technologies in colorectal cancer research are discussed.
结直肠癌通常在明确的阶段发展，每个阶段都有独特的分子改变特征。这个通常缓慢的过程使结直肠癌成为早期检测计划的理想目标。近年来，全球组学方法的蓬勃发展，极大地提高了对新型诊断和预后工具的期望。尽管早期有许多令人失望的情况，导致人们持怀疑态度，但在癌症研究方面确实取得了令人兴奋的新进展。本文综述了目前可用于鉴定新型生物标志物和药物靶点的蛋白质组学工具，并概述了它们在致癌分子机制研究中的应用。重点介绍了新型样品制备方法、蛋白质分离和鉴定技术以及定量蛋白质组学的先进质谱工具。讨论了这些技术在结直肠癌研究中的最重要应用。
