[人类树突状细胞特异性细胞间黏附分子3结合非整合素表达与结核分枝杆菌的相关性]

[The correlation between dendritic cell-specific ICAM-3 grabbing nonintegrin expression and Mycobacterium tuberculosis in humans].

作者信息

Zhang Jie, Guo Shu-liang, Luo Yong-ai

机构信息

Department of Respiratory Medicine, First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2009 Aug;32(8):572-5.

PMID:19958673

Abstract

OBJECTIVE

To investigate the correlation between dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN) expression and Mycobacterium tuberculosis in humans.

METHODS

The peripheral blood mononuclear cells were obtained respectively from 25 patients with tuberculosis, 25 patients with pneumonia and 25 healthy individuals, and were cultured in medium with GM-CSF and IL-4. Five days later, expression of CD(11c), CD(86), HLA-DR and DC-SIGN were detected by flow cytometry. The expression of DC-SIGN mRNA was detected by RT-PCR. The levels of IL-12 and IL-10 in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Proliferation induction of T lymphocytes dendritic cells (DCs) was measured by mixed lymphocyte reaction (MLR).

RESULTS

The expression of CD(86) on DCs in the tuberculosis patients [(72 +/- 11)%] was significantly lower than those in the other 2 groups [(87 +/- 16)%, (92 +/- 6)%] (F = 11.97, P < 0.01). In patients with tuberculosis, the expression of DC-SIGN on DCs [(85 +/- 8)%] was significantly higher than the other 2 groups [(60 +/- 28)%, (62 +/- 13)%] (F = 8.27, P < 0.01), and so was the DC-SIGN mRNA level (F = 3.99, P < 0.05). The levels of IL-10 secreted by DCs in the tuberculosis patients [(98 +/- 31) ng/L] were significantly higher than those in the other 2 groups [(74 +/- 38) ng/L and (66 +/- 27) ng/L] (F = 4.19, P < 0.05). MLR showed lower potency of proliferation induction of T lymphocytes in the tuberculosis patients (1858 +/- 628) than the other 2 groups (3066 +/- 1389), (3383 +/- 1163) (F = 7.92, P < 0.01). Expression of CD(11c) and HLA-DR on DCs and IL-12 levels were not significantly different among the 3 groups (P > 0.05).

CONCLUSION

The decreased DC maturity, elevated IL-10 secretion by DCs, decreased proliferation induction of T lymphocytes and impaired immune response may be due to overexpression of DC-SIGN in patients with tuberculosis.

摘要

目的

探讨树突状细胞特异性细胞间黏附分子3抓取非整合素（DC-SIGN）表达与人类结核分枝杆菌之间的相关性。

方法

分别从25例结核病患者、25例肺炎患者和25名健康个体中获取外周血单个核细胞，并在含粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4（IL-4）的培养基中培养。5天后，采用流式细胞术检测CD(11c)、CD(86)、人白细胞抗原DR（HLA-DR）和DC-SIGN的表达。采用逆转录聚合酶链反应（RT-PCR）检测DC-SIGN mRNA的表达。采用酶联免疫吸附测定（ELISA）法检测上清液中白细胞介素-12（IL-12）和白细胞介素-10（IL-10）的水平。采用混合淋巴细胞反应（MLR）检测树突状细胞（DCs）对T淋巴细胞的增殖诱导作用。

结果

结核病患者DCs上CD(86)的表达[(72±11)%]显著低于其他两组[(87±16)%，(92±6)%]（F = 11.97，P < 0.01）。结核病患者DCs上DC-SIGN的表达[(85±8)%]显著高于其他两组[(60±28)%，(62±13)%]（F = 8.27，P < 0.01），DC-SIGN mRNA水平也是如此（F = 3.99，P < 0.05）。结核病患者DCs分泌的IL-10水平[(98±31) ng/L]显著高于其他两组[(74±38) ng/L和(66±27) ng/L]（F = 4.19，P < 0.05）。MLR显示，结核病患者T淋巴细胞的增殖诱导能力（1858±628）低于其他两组（3066±1389），（3383±1163）（F = 7.92，P < 0.01）。3组间DCs上CD(11c)和HLA-DR的表达及IL-12水平差异无统计学意义（P > 0.05）。