Department of Respiratory Medicine, The First Affiliated Hospital, Chongqing Medical University, No. 1 You Yi Road, Chongqing, China.
Cell Immunol. 2011;272(1):94-101. doi: 10.1016/j.cellimm.2011.09.001. Epub 2011 Sep 21.
The aim of the study was to investigate the interaction between manLAM and DC-SIGN influencing DCs maturation and downstream immune response using small interfering RNA-expressing lentiviral vectors to specifically knockdown DC-SIGN. Our data indicated that DC-SIGN knockdown alone in DCs did not affect the maturation or the immunological function of lipopolysacharide (LPS)-activated DCs. Surface molecules were dramatically down-regulated in DCs primed with manLAM but not in mock control DCs (P<0.05). Meanwhile, manLAM enhanced the production of the immunosuppressive cytokine IL-10 in DCs (P<0.05). The level of IFN-γ was significantly down-regulated in the supernatants of naive T cells after co-cultured with DCs primed with manLAM (P<0.05). We demonstrated that DCs primed with manLAM may partially impair maturation phenotypes and immune response in LPS-activated DCs. However, the alterations of DCs function and downstream immune response caused by manLAM were reversed by the knockdown of DC-SIGN.
本研究旨在通过表达小干扰 RNA 的慢病毒载体特异性敲低 DC-SIGN，研究甘露糖型纤连蛋白（manLAM）与 DC-SIGN 之间的相互作用对树突状细胞（DCs）成熟及下游免疫反应的影响。我们的数据表明，单独敲低 DC-SIGN 并不影响脂多糖（LPS）激活的 DCs 的成熟或免疫功能。与模拟对照 DCs 相比，manLAM 预刺激的 DCs 表面分子显著下调（P<0.05）。同时，manLAM 增强了 DCs 中免疫抑制细胞因子 IL-10 的产生（P<0.05）。与 manLAM 预刺激的 DCs 共培养后，幼稚 T 细胞上清液中 IFN-γ 的水平显著下调（P<0.05）。我们证明，manLAM 预刺激的 DCs 可能部分损害 LPS 激活的 DCs 的成熟表型和免疫反应。然而，manLAM 引起的 DC 功能和下游免疫反应的改变可通过敲低 DC-SIGN 逆转。
