National Institute of Malaria Research, FS-Jabalpur, MP, India.
Acta Trop. 2010 Mar;113(3):263-8. doi: 10.1016/j.actatropica.2009.11.009. Epub 2009 Dec 1.
The aim of this preliminary study was to investigate the plasma cytokine profiles in a group of patients suffering from Plasmodium vivax malaria during the peak of its transmission season. Plasma samples of 173 P. vivax patients and 34 healthy individuals were analyzed for IFN-gamma, TNF-alpha, IL-10 and IP-10 levels by ELISA. Levels of both pro- and anti-inflammatory cytokines were significantly higher in P. vivax patients compared to controls. Children with P. vivax infection had significantly higher levels of IFN-gamma than adults (P=0.017). Asexual parasitaemia versus TNF-alpha (r=-0.31, P=0.01), IL-10 (r=-0.30, P=0.015) and gametocytaemia versus IFN-gamma (r=-0.26; P=0.034) levels showed significant negative correlation in children compared to adults. The median concentrations of IFN-gamma (P=0.001), IL-10 (P=0.032) and IP-10 (P</=0.05) were higher in children reported with chills and rigors, whereas in adults only IFN-gamma levels was higher (P<0.0001). The median plasma concentrations of IFN- gamma (P=0.02), IL-10 (P<0.0001) and IP-10 (P=0.068) were higher in patients with mild anaemia compared to non-anaemic patients. The results indicated that both pro- and anti-inflammatory cytokine responses are associated with clinical signs of mild anaemia and paroxysm during symptomatic P. vivax malaria in Central India.
本初步研究旨在调查在疟疾传播高峰期患有间日疟原虫疟疾的患者群体中的血浆细胞因子谱。通过 ELISA 分析了 173 例间日疟原虫患者和 34 名健康个体的血浆样本中 IFN-γ、TNF-α、IL-10 和 IP-10 的水平。与对照组相比，间日疟原虫患者的促炎和抗炎细胞因子水平均显著升高。感染间日疟原虫的儿童 IFN-γ水平明显高于成人（P=0.017）。与成人相比，儿童无环体血症与 TNF-α（r=-0.31，P=0.01）、IL-10（r=-0.30，P=0.015）和配子体血症与 IFN-γ（r=-0.26；P=0.034）水平呈显著负相关。与成人相比，发热和寒战的儿童 IFN-γ（P=0.001）、IL-10（P=0.032）和 IP-10（P</=0.05）的中位数浓度较高，而成人仅 IFN-γ水平较高（P<0.0001）。与非贫血患者相比，轻度贫血患者 IFN-γ（P=0.02）、IL-10（P<0.0001）和 IP-10（P=0.068）的血浆浓度中位数较高。结果表明，促炎和抗炎细胞因子反应与印度中部间日疟原虫疟疾有症状期的轻度贫血和发作的临床体征有关。
