Murino Rafacho Bruna Paola, Portugal Dos Santos Priscila, Gonçalves Andréa de Freitas, Fernandes Ana Angélica Henrique, Okoshi Katashi, Chiuso-Minicucci Fernanda, Azevedo Paula S, Mamede Zornoff Leonardo Antonio, Minicucci Marcos Ferreira, Wang Xiang-Dong, Rupp de Paiva Sergio Alberto
Internal Medicine Department, Botucatu Medical School-UNESP, Botucatu/SP, Brazil.
Department of Biochemistry, Botucatu Biosciences Institute-UNESP, Botucatu/SP, Brazil.
PLoS One. 2017 May 11;12(5):e0177521. doi: 10.1371/journal.pone.0177521. eCollection 2017.
Myocardial infarction (MI) is one of the leading causes of morbidity and mortality worldwide. Dietary intervention on adverse cardiac remodeling after MI has significant clinical relevance. Rosemary leaves are a natural product with antioxidant/anti-inflammatory properties, but its effect on morphology and ventricular function after MI is unknown.
To determine the effect of the dietary supplementation of rosemary leaves on cardiac remodeling after MI, male Wistar rats were divided into 6 groups after sham procedure or experimental induced MI: 1) Sham group fed standard chow (SR0, n = 23); 2) Sham group fed standard chow supplemented with 0.02% rosemary (R002) (SR002, n = 23); 3) Sham group fed standard chow supplemented with 0.2% rosemary (R02) (SR02, n = 22); 4) group submitted to MI and fed standard chow (IR0, n = 13); 5) group submitted to MI and fed standard chow supplemented with R002 (IR002, n = 8); and 6) group submitted to MI and fed standard chow supplemented with R02 (IR02, n = 9). After 3 months of the treatment, systolic pressure evaluation, echocardiography and euthanasia were performed. Left ventricular samples were evaluated for: fibrosis, cytokine levels, apoptosis, energy metabolism enzymes, and oxidative stress. Rosemary dietary supplementation attenuated cardiac remodeling by improving energy metabolism and decreasing oxidative stress. Rosemary supplementation of 0.02% improved diastolic function and reduced hypertrophy after MI. Regarding rosemary dose, 0.02% and 0.2% for rats are equivalent to 11 mg and 110 mg for humans, respectively.
Our findings support further investigations of the rosemary use as adjuvant therapy in adverse cardiac remodeling.
心肌梗死（MI）是全球发病和死亡的主要原因之一。心肌梗死后饮食干预对不良心脏重塑具有重要的临床意义。迷迭香叶是一种具有抗氧化/抗炎特性的天然产物，但其对心肌梗死后形态和心室功能的影响尚不清楚。
为了确定饮食补充迷迭香叶对心肌梗死后心脏重塑的影响，将雄性Wistar大鼠在假手术或实验性诱导心肌梗死后分为6组：1）喂食标准饲料的假手术组（SR0，n = 23）；2）喂食添加0.02%迷迭香的标准饲料的假手术组（R002）（SR002，n = 23）；3）喂食添加0.2%迷迭香的标准饲料的假手术组（R02）（SR02，n = 22）；4）接受心肌梗死并喂食标准饲料的组（IR0，n = 13）；5）接受心肌梗死并喂食添加R002的标准饲料的组（IR002，n = 8）；6）接受心肌梗死并喂食添加R02的标准饲料的组（IR02，n = 9）。治疗3个月后，进行收缩压评估、超声心动图检查和安乐死。对左心室样本进行以下评估：纤维化、细胞因子水平、细胞凋亡、能量代谢酶和氧化应激。饮食补充迷迭香叶通过改善能量代谢和降低氧化应激减轻心脏重塑。补充0.02%的迷迭香可改善心肌梗死后的舒张功能并减轻肥大。关于迷迭香剂量，大鼠的0.02%和0.2%分别相当于人类的11毫克和110毫克。
我们的研究结果支持进一步研究将迷迭香用作不良心脏重塑辅助治疗的可能性。
