National Genetics Reference Laboratory (Wessex), Salisbury NHS Foundation Trust, Salisbury SP2 8BJ, UK.
Best Pract Res Clin Haematol. 2009 Sep;22(3):355-65. doi: 10.1016/j.beha.2009.04.001.
Molecular monitoring of chronic myeloid leukaemia (CML) patients by real time quantitative reverse transcriptase PCR (RQ-PCR) is of clinical value, but the use of diverse laboratory protocols and units of measurement make it difficult to compare results between and sometimes within centres. This review explores the intrinsic difficulties in standardising the RQ-PCR analysis, summarises the progress that has been made following the proposal for a new International Scale for BCR-ABL measurement and discusses how further improvements are likely to be made.
实时荧光定量逆转录聚合酶链反应（RQ-PCR）对慢性髓系白血病（CML）患者进行分子监测具有重要的临床价值，但是不同实验室方案和测量单位的使用使得很难在不同中心之间甚至是同一中心内部比较结果。本文探讨了 RQ-PCR 分析标准化的固有困难，总结了在提出新的 BCR-ABL 测量国际标准后取得的进展，并讨论了如何进一步改进。
