School of Pharmacy, Yantai University, 32# Qingquan Road, Laishan District, Yantai 264003, PR China.
Phytomedicine. 2010 Jul;17(8-9):635-9. doi: 10.1016/j.phymed.2009.10.017. Epub 2009 Dec 2.
The present study was undertaken to examine the effect of forsythoside B (FB) on rat myocardial ischemia-reperfusion (I/R) model and elucidate the potential mechanism. Left ventricular systolic pressure (LVSP) and +/-dp/dt(max) were detected. Blood samples were collected to determine serum levels of troponin T (Tn-T), TNF-alpha and IL-6. Hearts were harvested to assess histopathological change and infarct size, determine content of MDA, myeloperoxidase (MPO), SOD and GPx activities, analyze expression of high-mobility group box 1 (HMGB1), phosphor-I kappaB-alpha and phosphor-nuclear factor kappaB (NF-kappaB) in ischemic myocardial tissue by Western blot. Compared with control group, rats treatment with FB showed a significant recovery in myocardial function with improvement of LVSP and +/-dp/dt(max). The myocardial infarct volume, serum levels of Tn-T, TNF-alpha and IL-6, content of MDA and MPO activity in myocardial tissue were all reduced, protein expression of HMGB1, phosphor-I kappaB-alpha and phosphor-NF-kappaB were down-regulated, while attenuated the decrease of SOD and GPx activities. Besides, the infiltration of polymorph nuclear leukocytes (PMNs) and histopathological damages in myocardium were decreased in FB treated groups. These findings suggested that FB rescued cardiac function from I/R injury by limiting inflammation response and its antioxidant properties.
本研究旨在探讨连翘酯苷 B (FB) 对大鼠心肌缺血再灌注 (I/R) 模型的影响，并阐明其潜在机制。检测左心室收缩压 (LVSP) 和 +/-dp/dt(max)。采集血样以测定血清肌钙蛋白 T (Tn-T)、TNF-α 和 IL-6 水平。采集心脏组织评估组织病理学改变和梗死面积，测定丙二醛 (MDA)、髓过氧化物酶 (MPO)、超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GPx) 活性，Western blot 分析缺血心肌组织中高迁移率族蛋白 1 (HMGB1)、磷酸化-IκB-α 和磷酸化核因子 kappaB (NF-κB) 的表达。与对照组相比，FB 治疗组心肌功能明显恢复，LVSP 和 +/-dp/dt(max) 改善。心肌梗死体积、血清 Tn-T、TNF-α 和 IL-6 水平、MDA 含量和 MPO 活性均降低，HMGB1、磷酸化-IκB-α 和磷酸化-NF-κB 蛋白表达下调，SOD 和 GPx 活性降低。此外，FB 治疗组心肌中多形核白细胞 (PMN) 的浸润和组织病理学损伤减少。这些发现表明，FB 通过限制炎症反应及其抗氧化特性来挽救 I/R 损伤引起的心脏功能障碍。
