College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao 266003, PR China.
Phytomedicine. 2011 Feb 15;18(4):266-71. doi: 10.1016/j.phymed.2010.07.009. Epub 2010 Aug 23.
Cornuside is a secoiridoid glucoside isolated from the fruit of Cornus officinalis SIEB. et ZUCC. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effects of cornuside in vivo and elucidated the potential mechanism. Rat models of myocardial I/R were induced by coronary occlusion followed by reperfusion or by Isoproterenol (ISO), treatment of rats with cornuside (20 and 40 mg/kg, i.v.) protected the animals from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics and reduction of myocardial damage severity. Treatment with cornuside also attenuated polymorphonuclear leukocytes (PMNs) infiltration, decreased myeloperoxidase (MPO) activity in the heart, lowered serum levels of pro-inflammatory factors and reduced phosphorylated IκB-α and nuclear factor kappa B (NF-κB) levels in the heart. Additionally, cornuside was shown to have remarkable antioxidant activity and inhibited ISO-induced myocardial cell necrosis. Thus, cornuside appeared to protect the rat from myocardial I/R injury by acting as an anti-inflammatory agent. These findings suggested that cornuside may be used therapeutically in the setting of myocardial I/R where inflammation and oxidant injury are prominent.
山茱萸苷是从山茱萸果实中分离得到的一种环烯醚萜苷。本研究旨在探讨山茱萸苷在体内抗心肌缺血再灌注（I/R）损伤的作用及其潜在机制。通过冠状动脉结扎再灌注或异丙肾上腺素（ISO）处理大鼠诱导心肌 I/R 模型，山茱萸苷（20 和 40mg/kg，静脉注射）治疗可减轻心肌 I/R 损伤，表现为梗死面积减小、血液动力学改善和心肌损伤程度减轻。山茱萸苷治疗还可减轻多形核白细胞（PMN）浸润，降低心脏髓过氧化物酶（MPO）活性，降低血清促炎因子水平，并降低心脏中磷酸化 IκB-α和核因子 kappa B（NF-κB）水平。此外，山茱萸苷具有显著的抗氧化活性，可抑制 ISO 诱导的心肌细胞坏死。因此，山茱萸苷可能通过作为一种抗炎剂来保护大鼠免受心肌 I/R 损伤。这些发现表明，山茱萸苷在炎症和氧化损伤明显的心肌 I/R 情况下可能具有治疗作用。
