粗糙脉孢菌的烟酰胺腺嘌呤二核苷酸特异性谷氨酸脱氢酶。VIII. 马来酰化蛋白胰蛋白酶水解产物中肽段的分离与序列分析
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VIII. Isolation and sequences of peptides from a tryptic hydrolysate of the maleylated protein.
作者信息
Austen B M, Nyc J F, Brown D M, Smith E L
出版信息
J Biol Chem. 1977 Nov 25;252(22):8182-95.
PMID:199603
Abstract
The NAD-specific glutamate dehydrogenase of Neurospora crassa was S-carboxymethylated with [14C]iodoacetate, maleylated, and hydrolyzed with trypsin. The isolation and sequences of the resulting peptides are described. These peptides gave information on the structure of the protein that was previously unknown and gave many overlaps of previously isolated segments of the protein.
摘要
粗糙脉孢菌的NAD特异性谷氨酸脱氢酶用[14C]碘乙酸进行S-羧甲基化、马来酰化，并经胰蛋白酶水解。描述了所得肽段的分离和序列。这些肽段提供了关于该蛋白质结构的先前未知信息，并与先前分离的蛋白质片段有许多重叠。
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