Piper Jörg
Clinic Meduna, Bad Bertrich, D-56864, Bad Bertrich, Germany.
Methods Mol Biol. 2010;611:193-210. doi: 10.1007/978-1-60327-345-9_16.
Several software solutions are powerful tools to enhance the depth of field and improve focus in digital photomicrography. By these means, the focal depth can be fundamentally optimized so that three-dimensional structures within specimens can be documented with superior quality. Thus, images can be created in light microscopy which will be comparable with scanning electron micrographs. The remaining sharpness will no longer be dependent on the specimen's vertical dimension or its range in regional thickness. Moreover, any potential lack of definition associated with loss of planarity and unsteadiness in the visual accommodation can be mitigated or eliminated so that the contour sharpness and resolution can be strongly enhanced.Through the use of complementary software, ultrahigh ranges in brightness and contrast (the so-called high-dynamic range) can be corrected so that the final images will also be free from locally over- or underexposed zones. Furthermore, fine detail in low natural contrast can be visualized in much higher clarity. Fundamental enhancements of the global visual information will result from both techniques.
几种软件解决方案是增强数字显微摄影景深和改善对焦的强大工具。通过这些方法，焦深可从根本上得到优化，从而能够以卓越的质量记录标本内的三维结构。因此，光学显微镜下可创建出与扫描电子显微镜图像相媲美的图像。剩余的清晰度将不再取决于标本的垂直尺寸或其局部厚度范围。此外，与视觉调节中的平面度丧失和不稳定相关的任何潜在清晰度不足都可以得到缓解或消除，从而显著提高轮廓清晰度和分辨率。通过使用配套软件，可以校正超高范围的亮度和对比度（即所谓的高动态范围），使最终图像也不会出现局部曝光过度或曝光不足的区域。此外，低自然对比度下的精细细节能够以更高的清晰度呈现出来。这两种技术都将从根本上增强整体视觉信息。
