Tiekotter Kenneth L, Ackermann Hans-W
Department of Biology, University of Portland, Portland, OR 97203, USA.
J Virol Methods. 2009 Jul;159(1):87-92. doi: 10.1016/j.jviromet.2009.03.004. Epub 2009 Mar 17.
The introduction of digital cameras has led to the publication of numerous virus electron micrographs of low magnification, poor contrast, and low resolution. Described herein is the methodology for obtaining highly contrasted virus images in the magnification range of approximately 250,000-300,000x. Based on recent advances in charged couple device (CCD) digital camera technology, methodology is described for optimal imaging parameters for using CCD cameras mounted in side- and bottom-mount position of electron microscopes and the recommendation of higher accelerating voltages, larger objective apertures, and small spot size. The authors are concerned with the principles of image formation and modulation, advocate a better use of imaging software to improve image quality, and recommend either pre- or post-acquisition adjustment for distributing pixel intensities of compressed histograms over the entire range of tonal values.
数码相机的引入导致了大量低放大倍数、低对比度和低分辨率的病毒电子显微照片的发表。本文描述了在大约250,000-300,000倍放大范围内获得高对比度病毒图像的方法。基于电荷耦合器件（CCD）数码相机技术的最新进展，描述了使用安装在电子显微镜侧面和底部位置的CCD相机的最佳成像参数的方法，以及更高加速电压、更大物镜孔径和小光斑尺寸的建议。作者关注图像形成和调制的原理，主张更好地使用成像软件来提高图像质量，并建议在采集前或采集后进行调整，以将压缩直方图的像素强度分布在整个色调值范围内。
