Rusinova G G, Turdakova V A, Muskacheva G S
Med Radiol (Mosk). 1991;36(2):51-4.
The genome of the thymus and bone marrow of the Wistar rat brain was investigated during daily repeated irradiation at a dose of 0.5 Gy in total doses of 0.5-20 Gy. The genome was assessed by a number of tests characterizing the high and low levels of DNA structural organization. Analysis was performed one day after the last fraction of irradiation. Stable changes in the primary DNA structure of thymocytes were shown by a decrease in the content of double-strand DNA using spectrofluorimetry. Changes in the primary DNA structure of the bone marrow were undetectable. Prolonged irradiation led to the formation of conformation changes in the nucleoid of cells of both tissues detectable by viscosimetric and spectrofluorimetric methods.
在以0.5 Gy的剂量每日重复照射、总剂量为0.5 - 20 Gy的过程中，对Wistar大鼠脑的胸腺和骨髓基因组进行了研究。通过一系列表征DNA结构组织高低水平的测试来评估基因组。在最后一次照射部分后一天进行分析。使用荧光分光光度法显示，胸腺细胞初级DNA结构的稳定变化表现为双链DNA含量的降低。骨髓初级DNA结构的变化未被检测到。长时间照射导致两种组织细胞的类核构象发生变化，这可通过粘度测定法和荧光分光光度法检测到。
