Qin Hai-Yan, Suo Zhi-Rong, Liu Wen-Zhe
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
Zhong Yao Cai. 2009 Aug;32(8):1199-201.
To determine the content of total salvianolic acid in different vegetative organs of Salvia miltiorrhiza and discover the dynamic change rules of tanshinol, protocatechuic aldehyde, caffeic acid and total salvianolic acid during the whole process of grwth.
HPLC-ECD was used. The separation was performed on Zorbax SB-C18 (150 mm x 4.6 mm, 5.0 microm) column by gradient elution. The mobile phase consisted of CH3OH-0.4% aqueous phosphoric acid. The flow rate was 1.0 mL/min. The detection was done at 0.7 V and the column temperature was 30 degrees C.
The highest content of total salvianolic acid in leaf was in June and gradually dropped off till the lowest in December; The content of total salvianolic acid in leaf gradually decreased along with the growing of the leaf. The content of total salvianolic acid in root was high and consistent from July to September, but gradually dropped off till the lowest in November.
The leaves of Salvia miltiorrhiza can be harvested in strong growth period to achieve the comprehensive use of the herb.
测定丹参不同营养器官中总丹参酚酸的含量，探索丹参素、原儿茶醛、咖啡酸及总丹参酚酸在其生长全过程中的动态变化规律。
采用高效液相色谱 - 电化学检测法（HPLC - ECD）。在Zorbax SB - C18（150 mm×4.6 mm，5.0微米）色谱柱上进行梯度洗脱分离。流动相为甲醇 - 0.4%磷酸水溶液。流速为1.0 mL/min。检测电压为0.7 V，柱温为30℃。
叶片中总丹参酚酸含量6月最高，随后逐渐下降，至12月降至最低；叶片中总丹参酚酸含量随叶片生长逐渐降低。根中总丹参酚酸含量7至9月较高且稳定，但11月逐渐下降至最低。
丹参叶片可在生长旺盛期采收，以实现该药材的综合利用。
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