Wang Juan, Zhang Qiu-Ye, Chen Yong-Xing
Department of Pediatrics, the Affiliated Hospital of Medical College, Qingdao University, Shandong.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Sep;29(9):794-7.
To explore the effects of Astragalus membranaceus (AM) on the cytokines secretion of peripheral dendritic cells (DC), including interleukin-10, -12, and -18 (IL-10, IL-12 and IL-18), in children with Henoch-Schonlein purpura (HSP) in the acute phase; and to study the immunological regulation mechanism of AM.
Peripheral blood mononuclear cells (PBMC) were obtained from 28 children with acute HSP by density gradient centrifugation, and each sample was divided into two parts, one untreated and one treated with AM. All cells were developed to mature DC through treating with recombinant human granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha). Expression of CD83 in the surface of mature DC was detected by flow cytometry, and levels of IL-10, IL-12 and IL-18 in the supernatant were measured by ELISA.
The supernatant level of IL-12 was higher [(141.58 +/- 100.19) ng/L vs (96.18 +/- 76.65) ng/L, t = 3.90, P<0.01], while levels of IL-10 and IL-18 were lower (t = 2.70, P<0.05; t = 4.07, P<0.01) in AM treated PBMCs than those in the untreated ones.
AM can correct the immunologic dysfunction of HSP children through increasing the IL-12, and decreasing the IL-10 and IL-18 secretions of PBMCs.
探讨黄芪对急性期过敏性紫癜（HSP）患儿外周血树突状细胞（DC）细胞因子分泌的影响，包括白细胞介素-10、-12和-18（IL-10、IL-12和IL-18）；并研究黄芪的免疫调节机制。
通过密度梯度离心法从28例急性HSP患儿中获取外周血单个核细胞（PBMC），每个样本分为两部分，一部分不做处理，另一部分用黄芪处理。所有细胞经重组人粒细胞巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）处理后发育为成熟DC。采用流式细胞术检测成熟DC表面CD83的表达，采用酶联免疫吸附测定法（ELISA）检测上清液中IL-10、IL-12和IL-18的水平。
黄芪处理的PBMC中IL-12的上清液水平较高[（141.58±100.19）ng/L比（96.18±76.65）ng/L，t = 3.90，P<0.01]，而IL-10和IL-18的水平较低（t = 2.70，P<0.05；t = 4.07，P<0.01）。
黄芪可通过增加IL-12、降低PBMC中IL-10和IL-18的分泌来纠正HSP患儿的免疫功能紊乱。
