Ag Biotech LLC, 1704 Cordell Drive, Tallahassee, FL 32303, USA.
Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322, USA.
J Biol Eng. 2009 Dec 4;3:20. doi: 10.1186/1754-1611-3-20.
Silver has long been known to have antimicrobial activity. To incorporate this property into multiple applications, a silver-impregnated cellulose (SIC) with low cytotoxicity to human cells was developed. SIC differs from other silver treatment methods in that the leaching of silver particles is non-existent and the release of ionic silver is highly controlled.
Candida albicans, Micrococcus luteu, Pseudomonas putida, and Escherichia coli were used for antimicrobial testing. No microbial cells were able to grow in the presence of SIC at concentrations above 0.0035 Ag w/v %. Even at a concentration of 0.00035 Ag w/v %, P. putida and M. luteu failed to grow, and C. albicans and E. coli exhibited diminished growth. To determine the cytotoxic effect of silver on human cells, five different concentrations of SIC were tested on human fibroblasts. In SIC concentrations of 0.035 Ag w/v % and below, no cytotoxicity was observed.
The optimal concentration of SIC for a broad range of anti-microbial activity and low or negligible cytotoxicity was 0.0035 Ag w/v %. Although the highly controlled releasing characteristics of SIC would prove a substantial improvement over current technologies, further investigation for genotoxicity and other biocompatibility test will be required.
银的抗菌活性早已为人所知。为了将这一特性应用于多种用途，开发了一种对人体细胞低细胞毒性的载银纤维素（SIC）。SIC 与其他银处理方法的不同之处在于不存在银颗粒的浸出，并且对离子银的释放有高度的控制。
使用白色念珠菌、藤黄微球菌、铜绿假单胞菌和大肠杆菌进行抗菌测试。在 SIC 浓度高于 0.0035Ag w/v%的情况下，没有微生物细胞能够生长。即使 SIC 浓度为 0.00035Ag w/v%，铜绿假单胞菌和藤黄微球菌也无法生长，而白色念珠菌和大肠杆菌的生长则受到抑制。为了确定银对人体细胞的细胞毒性作用，将五种不同浓度的 SIC 用于人成纤维细胞。在 SIC 浓度为 0.035Ag w/v%及以下时，未观察到细胞毒性。
对于广谱抗菌活性和低或可忽略不计的细胞毒性，SIC 的最佳浓度为 0.0035Ag w/v%。尽管 SIC 的高度受控释放特性将证明是对现有技术的重大改进，但仍需要进一步研究其遗传毒性和其他生物相容性测试。
Zotero 插件
