Cellular Neuroscience Laboratory, Biomedical Engineering, College of Engineering and Science, Louisiana Tech University, Ruston, LA 71270, USA.
Materials Science Institute of Madrid (ICMM), CSIC, 28049 Madrid, Spain.
Int J Environ Res Public Health. 2018 Apr 25;15(5):844. doi: 10.3390/ijerph15050844.
We previously described the novel synthesis of a copper high-aspect ratio structure (CuHARS) biohybrid material using cystine. While extremely stable in water, CuHARS is completely (but slowly) degradable in cellular media. Here, integration of the CuHARS into cellulose matrices was carried out to provide added control for CuHARS degradation. Synthesized CuHARS was concentrated by centrifugation and then dried. The weighed mass was re-suspended in water. CuHARS was stable in water for months without degradation. In contrast, 25 μg/mL of the CuHARS in complete cell culture media was completely degraded (slowly) in 18 days under physiological conditions. Stable integration of CuHARS into cellulose matrices was achieved through assembly by mixing cellulose micro- and nano-fibers and CuHARS in an aqueous (pulp mixture) phase, followed by drying. Additional materials were integrated to make the hybrids magnetically susceptible. The cellulose-CuHARS composite films could be transferred, weighed, and cut into usable pieces; they maintained their form after rehydration in water for at least 7 days and were compatible with cell culture studies using brain tumor (glioma) cells. These studies demonstrate utility of a CuHARS-cellulose biohybrid for applied applications including: (1) a platform for biomedical tracking and (2) integration into a 2D/3D matrix using natural products (cellulose).
我们之前描述了使用胱氨酸合成新型铜高纵横比结构（CuHARS）生物杂化材料的方法。虽然 CuHARS 在水中极其稳定，但它在细胞培养基中完全（但缓慢）可降解。在这里，将 CuHARS 整合到纤维素基质中，以提供对 CuHARS 降解的额外控制。通过离心浓缩合成的 CuHARS，然后干燥。称重后的质量重新悬浮在水中。CuHARS 在水中数月稳定而不会降解。相比之下，在生理条件下，25μg/mL 的完整细胞培养基中的 CuHARS 在 18 天内完全（缓慢）降解。通过在水相（纸浆混合物）中将纤维素微纤维和纳米纤维与 CuHARS 混合进行组装，然后干燥，实现了 CuHARS 到纤维素基质的稳定整合。添加了其他材料以使其具有磁性敏感性。纤维素-CuHARS 复合薄膜可以转移、称重并切成可用的小块；它们在水中重新水合后至少保持 7 天的形状，并且与脑肿瘤（神经胶质瘤）细胞的细胞培养研究兼容。这些研究证明了 CuHARS-纤维素生物杂化材料在应用方面的实用性，包括：（1）用于生物医学追踪的平台，以及（2）使用天然产物（纤维素）整合到 2D/3D 基质中。
