用于普鲁兰多糖纯化的包含聚乙二醇和葡聚糖的双水相系统的开发：相图与经济分析。
Development of aqueous two-phase systems comprising poly ethylene glycol and dextran for purification of pullulan: Phase diagrams and fiscal analysis.
作者信息
Badhwar Parul, Kumar Punit, Dubey Kashyap Kumar
机构信息
Microbial Process Development Laboratory University Institute of Engineering and Technology Maharshi Dayanand University Rohtak Haryana India.
Department of Biotechnology Central University of Haryana Mahendergarh Haryana India.
出版信息
Eng Life Sci. 2018 May 24;18(8):524-531. doi: 10.1002/elsc.201700156. eCollection 2018 Aug.
Pullulan is a commercially important Exopolysaccharide (EPS) with wide-spread applications which is produced by . The alternative α (1 4) & α (1 6) configuration in pullulan provides it the specific structural and conformational properties. Pullulan is currently being exploited in food, health care, pharmacy, lithography, cosmetics. The fermented broth is processed by organic solvent precipitation for isolation and purification of pullulan. In this study, we have tried to analyze the potential of aqueous two phase system as an alternate technique to extract pullulan from fermented broth. Including this viability of ATPS was also compared with conventional organic solvent precipitation system in terms of cost and time. It was found that ATPS process produced a higher yield of pullulan (80.56%) than organic solvent precipitation method (71.6%). ATPS was also found more economical and less time consuming method.
普鲁兰多糖是一种具有重要商业价值的胞外多糖（EPS），应用广泛，它由……产生。普鲁兰多糖中交替的α（1→4）和α（1→6）构型赋予了它特定的结构和构象性质。目前，普鲁兰多糖正在食品、医疗保健、制药、光刻、化妆品等领域得到应用。发酵液通过有机溶剂沉淀法进行处理，以分离和纯化普鲁兰多糖。在本研究中，我们试图分析双水相系统作为从发酵液中提取普鲁兰多糖的替代技术的潜力。此外，还在成本和时间方面将双水相系统的可行性与传统有机溶剂沉淀系统进行了比较。结果发现，双水相系统法生产的普鲁兰多糖产量（80.56%）高于有机溶剂沉淀法（71.6%）。还发现双水相系统法更经济且耗时更少。
Zotero 插件