Forest Biotechnology Division, Korea Forest Research Institute, 44-3 Omokchundong, Suwon 441-350, Republic of Korea.
Plant Physiol Biochem. 2010 Feb-Mar;48(2-3):136-41. doi: 10.1016/j.plaphy.2009.11.002. Epub 2009 Nov 18.
Osmotic stress induces changes in the expression of various genes including those associated with drought tolerance, cell wall metabolism and defense. We isolated 852 cDNA clones, the expression of which is induced by osmotic stress, from cells of a hybrid poplar (Populus alba x Populus tremula var. glandulosa) by suppression subtractive hybridization after mannitol treatment. We examined how stress affected their expression using cDNA microarray analysis, which identified 104 genes significantly up-regulated by osmotic stress. These include genes with functions related to transcription, signal transduction, cell wall metabolism and defense. Other gene transcripts encoding cysteine protease and aquaporin are also up-regulated during osmotic stress. The function of about one-third of the genes in poplar cells that were significantly up-regulated by stress is not known, suggesting that the cell suspension may offer an opportunity of finding novel genes otherwise never expressed and that we still need more information at the molecular level.
渗透胁迫诱导各种基因的表达发生变化，包括与耐旱性、细胞壁代谢和防御相关的基因。我们通过甘露醇处理后，利用抑制差减杂交从杂交杨（Populus alba x Populus tremula var. glandulosa）细胞中分离出 852 个 cDNA 克隆，这些克隆的表达受渗透胁迫诱导。我们使用 cDNA 微阵列分析来研究胁迫对它们表达的影响，结果鉴定出 104 个受渗透胁迫显著上调的基因。这些基因的功能与转录、信号转导、细胞壁代谢和防御有关。其他一些基因的转录本编码半胱氨酸蛋白酶和水通道蛋白，在渗透胁迫过程中也被上调。在胁迫下显著上调的杨树细胞中约三分之一的基因的功能尚不清楚，这表明细胞悬浮液可能提供了一个机会，可以发现以前从未表达过的新基因，我们仍然需要更多的分子水平的信息。
