Institute of Bioscience and Biotechnology, Daewoong Pharmaceutical Co. Ltd., Yongin, Gyeonggi-do 449-814, Republic of Korea.
Res Vet Sci. 2010 Jun;88(3):523-7. doi: 10.1016/j.rvsc.2009.11.007. Epub 2009 Dec 5.
Avian-lineage H3N2 canine influenza virus (CIV)-associated respiratory disease, which can be fatal, emerged in South Korean dogs in 2007. We show here that dogs experimentally infected with CIV only developed respiratory tract diseases, as no extrapulmonary lesions and virus antigens were detected. This differs from the multiorgan diseases that avian influenza H5N1 induces in small experimental animals. However, the CIV-infected dogs developed a distinctively severe, long-persistent bronchointerstitial pneumonia, which differs from the acute but short-term bronchopneumonia that human (H1N1 and H3N2) influenza cause in rodents and ferrets. Histopathology and in situ TUNEL assays revealed that the neutrophils infiltrating the lesions were undergoing apoptosis, which probably reflects the attempts by the body to maintain appropriate numbers of neutrophils for defense against secondary bacterial infections. Our observations suggest that neutrophils along with the related chemoattractant cytokines (TNF-alpha, IL-1 and IL-8, etc.) may play a key role in the pathogenesis of H3N2 CIV in dogs.
2007 年，韩国的犬只中出现了一种致命的禽流感 H3N2 犬流感病毒（CIV）相关呼吸道疾病。我们在此表明，实验感染 CIV 的犬只仅发展为呼吸道疾病，因为未检测到肺外病变和病毒抗原。这与禽流感 H5N1 在小型实验动物中引起的多器官疾病不同。然而，感染 CIV 的犬只发展出一种明显严重且持久的支气管间质性肺炎，与人类（H1N1 和 H3N2）流感在啮齿动物和雪貂中引起的急性但短期的支气管肺炎不同。组织病理学和原位 TUNEL 检测显示，浸润病变的中性粒细胞正在发生凋亡，这可能反映了机体试图维持适当数量的中性粒细胞来抵御继发细菌感染。我们的观察表明，中性粒细胞以及相关趋化因子细胞因子（TNF-α、IL-1 和 IL-8 等）可能在犬 H3N2 CIV 的发病机制中发挥关键作用。
