LABICAI/UFSC, Florianópolis, SC, Brazil.
Mar Environ Res. 2010;69 Suppl:S31-3. doi: 10.1016/j.marenvres.2009.11.002. Epub 2009 Nov 6.
Diesel fuel is a potential contaminant of estuarine and mangrove areas, particularly because it is the main fuel used in small boats and larger vessels. The aim of this work was to identify genes differentially expressed in the liver of Poecilia vivipara (Guppy) exposed to 10% diesel fuel water accommodated fraction (WAF), employing the subtractive suppressive hybridization (SSH) method. The results showed 27 differentially expressed gene fragments, 12 up-regulated and 15 down-regulated. Among the up-regulated genes were CYP1A, UDPGT1a, ABCC4, Methyltransferase and Apolipoprotein A1. Down-regulated genes included Vitellogenins, C1 Inhibitor and Complement Component 3c. The identified genes are associated with different metabolic functions like biotransformation, membrane transport and immune system, indicating the susceptibility and/or molecular responses of this organism to the toxic effects elicited by diesel fuel WSF.
柴油是港湾和红树林地区的潜在污染物，尤其是因为它是小船和大型船只使用的主要燃料。本工作的目的是使用消减抑制杂交（SSH）方法，鉴定暴露于 10%柴油水可容纳分数（WAF）的胎生孔雀鱼（孔雀鱼）肝脏中差异表达的基因。结果显示了 27 个差异表达的基因片段，12 个上调和 15 个下调。上调的基因包括 CYP1A、UDPGT1a、ABCC4、甲基转移酶和载脂蛋白 A1。下调的基因包括卵黄蛋白原、C1 抑制剂和补体成分 3c。鉴定出的基因与不同的代谢功能有关，如生物转化、膜转运和免疫系统，表明该生物对柴油 WSF 引起的毒性作用的易感性和/或分子反应。
