Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, NEPAQ, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Environ Toxicol Chem. 2012 Jun;31(6):1249-53. doi: 10.1002/etc.1837. Epub 2012 Apr 27.
Diesel fuel can cause adverse effects in marine invertebrates by mechanisms that are not clearly understood. The authors used suppressive subtractive hybridization to identify genes up- and downregulated in Crassostrea brasiliana exposed to diesel fuel. Genes putatively involved in protein regulation, innate immune, and stress response, were altered by diesel challenge. Three genes regulated by diesel were validated by quantitative real-time polymerase chain reaction. This study sheds light on transcriptomic responses of oysters to diesel pollution.
柴油机燃料通过尚未明确的机制对海洋无脊椎动物产生不良影响。作者使用抑制性消减杂交技术鉴定了暴露于柴油机燃料的巴西牡蛎中上调和下调的基因。柴油机胁迫改变了推测与蛋白质调控、先天免疫和应激反应相关的基因。通过定量实时聚合酶链反应验证了柴油机调节的三个基因。本研究阐明了牡蛎对柴油机污染的转录组反应。
