Yu Zhe, Elkin Benjamin S, Morrison Barclay
Department of Biomedical Engineering at Columbia University, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:1135-8. doi: 10.1109/IEMBS.2009.5332381.
Traumatic brain injury (TBI) is caused by mechanical forces, producing tissue deformation at the moment of injury. Complex cellular, neurochemical and metabolic alterations are initiated by the deformation and result in delayed cell death and dysfunction. Using an in vitro model of TBI based on organotypic brain slice cultures, we have quantitatively studied the relationship between tissue deformation and functional outcome. Specifically, we studied the effects of low levels of tissue deformation on the functional outcomes as measured by electrophysiology recordings. In response to 5% and 10% biaxial Lagrangian strain, the maximal evoked response and the excitability of neural networks were found to be decreased. Additionally, the different anatomic subregions of the hippocampus displayed different levels of impairment to the injuries. These results suggest that the network function was affected by low levels of applied strain which induced minimal cell death in previous studies.
创伤性脑损伤（TBI）由机械力引起，在损伤瞬间产生组织变形。这种变形引发复杂的细胞、神经化学和代谢改变，导致细胞延迟死亡和功能障碍。我们使用基于器官型脑片培养的TBI体外模型，定量研究了组织变形与功能结果之间的关系。具体而言，我们通过电生理记录研究了低水平组织变形对功能结果的影响。在5%和10%双轴拉格朗日应变作用下，发现最大诱发反应和神经网络兴奋性降低。此外，海马体的不同解剖亚区域对损伤表现出不同程度的损害。这些结果表明，在先前研究中诱导最小细胞死亡的低水平施加应变会影响网络功能。
