Institute of Neurobiology, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University Health Science Center, School of Basic Medical Sciences, Xi'an 710061, China.
Department of Physiology, Shandong Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, School of Basic Medicine, Qingdao University, Qingdao 266071, China.
Int J Mol Sci. 2023 Feb 20;24(4):4182. doi: 10.3390/ijms24044182.
Therapeutic strategies based on neural stem cells (NSCs) transplantation bring new hope for neural degenerative disorders, while the biological behaviors of NSCs after being grafted that were affected by the host tissue are still largely unknown. In this study, we engrafted NSCs that were isolated from a rat embryonic cerebral cortex onto organotypic brain slices to examine the interaction between grafts and the host tissue both in normal and pathological conditions, including oxygen-glucose deprivation (OGD) and traumatic injury. Our data showed that the survival and differentiation of NSCs were strongly influenced by the microenvironment of the host tissue. Enhanced neuronal differentiation was observed in normal conditions, while significantly more glial differentiation was observed in injured brain slices. The process growth of grafted NSCs was guided by the cytoarchitecture of host brain slices and showed the distinct difference between the cerebral cortex, corpus callosum and striatum. These findings provided a powerful resource for unraveling how the host environment determines the fate of grafted NSCs, and raise the prospect of NSCs transplantation therapy for neurological diseases.
基于神经干细胞（NSCs）移植的治疗策略为神经退行性疾病带来了新的希望，然而，移植后 NSCs 的生物学行为如何受到宿主组织的影响在很大程度上仍然未知。在这项研究中，我们将从大鼠胚胎大脑皮层分离出的 NSCs 移植到器官型脑片上，以在正常和病理条件下（包括氧葡萄糖剥夺（OGD）和创伤损伤）研究移植物与宿主组织之间的相互作用。我们的数据表明，NSCs 的存活和分化受到宿主组织微环境的强烈影响。在正常情况下观察到增强的神经元分化，而在受损脑片中观察到明显更多的神经胶质分化。移植的 NSCs 的生长过程受到宿主脑片细胞结构的指导，并且在大脑皮层、胼胝体和纹状体之间显示出明显的差异。这些发现为揭示宿主环境如何决定移植的 NSCs 的命运提供了有力的资源，并为神经疾病的 NSCs 移植治疗带来了前景。
