Kalghatgi Sameer U, Fridman Alexander, Friedman Gary, Clyne Alisa Morss
Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:6030-3. doi: 10.1109/IEMBS.2009.5333168.
Non-thermal dielectric barrier discharge plasma is currently being developed for a wide range of medical applications, including blood coagulation, malignant cell apoptosis, and wound healing. However, the effect of non-thermal plasma on the vasculature is unclear. Blood vessels are affected during plasma treatment of many tissues, and vessels themselves may be an important clinical plasma therapy target. We investigated the effect of non-thermal plasma treatment on endothelial cells, which line the inner surface of blood vessels. Non-thermal plasma treatment at short exposures (up to 30 seconds; 4 J/cm(2)) was relatively non-toxic to endothelial cells. Endothelial cells treated with plasma for 30 seconds demonstrated twice as much proliferation as untreated cells five days after plasma treatment. Proliferation was abrogated by a fibroblast growth factor-2 neutralizing antibody and reactive oxygen species inhibitors. This suggests that plasma-induced endothelial cell proliferation is caused by growth factor release following reactive oxygen species cell membrane damage. These data suggest that low power non-thermal plasma treatment is a potential novel therapy for promotion of endothelial cell mediated angiogenesis.
非热介质阻挡放电等离子体目前正被开发用于广泛的医学应用，包括血液凝固、恶性细胞凋亡和伤口愈合。然而，非热等离子体对脉管系统的影响尚不清楚。在许多组织的等离子体治疗过程中血管会受到影响，并且血管本身可能是临床等离子体治疗的一个重要靶点。我们研究了非热等离子体处理对血管内表面衬里的内皮细胞的影响。短时间暴露（长达30秒；4 J/cm²）的非热等离子体处理对内皮细胞相对无毒。经等离子体处理30秒的内皮细胞在等离子体处理五天后显示出的增殖是未处理细胞的两倍。增殖被成纤维细胞生长因子-2中和抗体和活性氧抑制剂消除。这表明等离子体诱导的内皮细胞增殖是由活性氧导致细胞膜损伤后生长因子释放引起的。这些数据表明低功率非热等离子体处理是促进内皮细胞介导的血管生成的一种潜在新疗法。
