液相放电等离子体对人体皮肤模型中伤口感染的细菌灭活作用。

Bacterial inactivation of wound infection in a human skin model by liquid-phase discharge plasma.

机构信息

Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado, United States of America.

出版信息

PLoS One. 2011;6(8):e24104. doi: 10.1371/journal.pone.0024104. Epub 2011 Aug 29.

BACKGROUND

We investigate disinfection of a reconstructed human skin model contaminated with biofilm-formative Staphylococcus aureus employing plasma discharge in liquid.

PRINCIPAL FINDINGS

We observed statistically significant 3.83-log10 (p<0.001) and 1.59-log10 (p<0.05) decreases in colony forming units of adherent S. aureus bacteria and 24 h S. aureus biofilm culture with plasma treatment. Plasma treatment was associated with minimal changes in histological morphology and tissue viability determined by means of MTT assay. Spectral analysis of the plasma discharge indicated the presence of highly reactive atomic oxygen radicals (777 nm and 844 nm) and OH bands in the UV region. The contribution of these and other plasma-generated agents and physical conditions to the reduction in bacterial load are discussed.

CONCLUSIONS

These findings demonstrate the potential of liquid plasma treatment as a potential adjunct therapy for chronic wounds.

背景

我们研究了液体等离子体放电对生物膜形成的金黄色葡萄球菌污染的重建人体皮肤模型的消毒效果。

主要发现

我们观察到，经过等离子体处理后，粘附的金黄色葡萄球菌细菌的菌落形成单位和 24 小时金黄色葡萄球菌生物膜培养物分别显著减少了 3.83-log10（p<0.001）和 1.59-log10（p<0.05）。等离子体处理与通过 MTT 测定法确定的组织形态学和组织活力的最小变化相关。等离子体放电的光谱分析表明存在高反应性的原子氧自由基（777nm 和 844nm）和紫外线区域中的 OH 带。讨论了这些和其他等离子体产生的试剂以及物理条件对减少细菌负荷的贡献。