Truxal Steven C, Huang Nien-Tsu, Kurabayashi Katsuo
Department of Mechanical Engineering, University of Michigan, MI, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:6693-5. doi: 10.1109/IEMBS.2009.5333287.
We report a microelectromechanical (MEMS) tunable optical filter and its integration in a fluorescence microscope for high speed on-chip spectral measurements. This integration allows for measurements of any fluorescence sample placed onto the microscope stage. We demonstrate the system capabilities by taking spectral measurements of multicolor fluorescent beads and fluorescently labeled cells passing through a microfluidic cytometer. The system has applications in biological studies where the measurement of multiple fluorescent peaks is restricted by the detection method's speed and sensitivity.
我们报道了一种微机电系统（MEMS）可调谐光学滤波器及其在荧光显微镜中的集成，用于高速片上光谱测量。这种集成使得能够对放置在显微镜载物台上的任何荧光样品进行测量。我们通过对穿过微流控细胞仪的多色荧光珠和荧光标记细胞进行光谱测量，展示了该系统的能力。该系统在生物学研究中具有应用价值，在这些研究中，多种荧光峰的测量受到检测方法速度和灵敏度的限制。
