从具有极强砷抗性的微杆菌 A33 菌株中鉴定砷基因簇。
Characterization of the ars gene cluster from extremely arsenic-resistant Microbacterium sp. strain A33.
机构信息
IEBE, UMR 7146 CNRS Université Paul Verlaine-Metz, Campus Bridoux, Rue du Général Delestraint, 57070 Metz, France.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):948-55. doi: 10.1128/AEM.01738-09. Epub 2009 Dec 4.
Abstract
The arsenic resistance gene cluster of Microbacterium sp. A33 contains a novel pair of genes (arsTX) encoding a thioredoxin system that are cotranscribed with an unusual arsRC2 fusion gene, ACR3, and arsC1 in an operon divergent from arsC3. The whole ars gene cluster is required to complement an Escherichia coli ars mutant. ArsRC2 negatively regulates the expression of the pentacistronic operon. ArsC1 and ArsC3 are related to thioredoxin-dependent arsenate reductases; however, ArsC3 lacks the two distal catalytic cysteine residues of this class of enzymes.
摘要
该砷抗性基因簇的 Microbacterium sp. A33 包含一对新的基因（arsTX）编码一个硫氧还蛋白系统，这是与一个不寻常的 arsRC2 融合基因 ACR3 和 arsC1 在一个操纵子转录的 divergent 从 arsC3。整个 ars 基因簇是需要补充大肠杆菌 ars 突变体。arsRC2 负调控 pentacistronic 操纵子的表达。arsC1 和 ArsC3 与依赖于硫氧还蛋白的砷酸盐还原酶有关；然而，ArsC3 缺乏该酶类的两个远端催化半胱氨酸残基。
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