Department of Cardiovascular Medicine, Akita University Graduate School of Medicine, Japan.
Circ J. 2010 Jan;74(1):203-6. doi: 10.1253/circj.cj-09-0589. Epub 2009 Dec 5.
The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice.
We generated transgenic mice expressing mCherry-LC3 under alphaMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation.
This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206).
自噬在心脏病中的作用已有报道。表达 GFP-LC3 的转基因小鼠已成为系统性检测自噬体的有用工具。使用 GFP-LC3 小鼠，很难区分心脏中自噬体形成增加与自噬体清除减少。
我们生成了在 alphaMyHC 启动子下表达 mCherry-LC3 的转基因小鼠，并将这些小鼠与表达 GFP-LC3 的转基因小鼠杂交。GFP 和 mCherry 对酸性条件的抗性差异克服了这一限制。
这种方法是一种创新性的方法，可以用于研究自噬的作用，并分析心肌细胞中自噬体的成熟。（Circ J 2010; 74: 203 - 206）。
