Deepa D, Jayakumari B, Thomas Sanjeev V
Kerala Registry of Epilepsy and Pregnancy, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Ann Indian Acad Neurol. 2008 Jan;11(1):44-6. doi: 10.4103/0972-2327.40225.
Lipid peroxidation is an indicator of free radical metabolism and oxidative stress in human beings and other organisms. Malondialdehyde (MDA), an end product of lipid peroxidation, is a metabolite that can be readily estimated in serum samples. Excess oxidative stress may be a final common pathway through which anti epileptic drugs may exert their teratogenic potential in pregnant women with epilepsy. Our objective in this study was to ascertain the variations in malondialdehyde (MDA) in women with epilepsy.
This study was carried out in the Kerala Registry of Epilepsy and pregnancy after obtaining clearance from the Institutional Ethics Committee. Informed consent was obtained from all the subjects. The quantitative examination of MDA was performed according to standard procedures. The ideal plasma level of MDA is below 2 nmol/ml.
Fifteen women with confirmed epilepsy (mean age 26.9 +/- 3.5) were included in the study. Two women were pregnant. MDA levels ranged from 1.7 to 2.8 nmol/ml (mean level = 2.13 +/- 0.37 nmol/ml). Eight women (53 %) had MDA levels above the upper limit of normal. Three patients had levels above 2.5 nmol/ml, which corresponded to the 75 centile.
This study had shown that the estimation of MDA levels in plasma is a convenient method to study lipid peroxidation and thereby oxidative stress in women with epilepsy. Over half of Women With Epilepsy (WWE) have excess oxidative stress as indicated by high levels of MDA in the plasma. Correlations between MDA level and characteristics of epilepsy, AED therapy, nutritional status and other medical conditions need to be observed in a larger cohort.
脂质过氧化是人类和其他生物体自由基代谢及氧化应激的一个指标。丙二醛（MDA）作为脂质过氧化的终产物，是一种可在血清样本中轻松测定的代谢物。过量的氧化应激可能是抗癫痫药物在癫痫孕妇中发挥致畸潜力的最终共同途径。我们在本研究中的目的是确定癫痫女性丙二醛（MDA）的变化情况。
本研究在获得机构伦理委员会批准后，于喀拉拉邦癫痫与妊娠登记处开展。所有受试者均签署了知情同意书。MDA的定量检测按照标准程序进行。MDA的理想血浆水平低于2纳摩尔/毫升。
15名确诊癫痫的女性（平均年龄26.9±3.5岁）纳入本研究。两名女性为孕妇。MDA水平在1.7至2.8纳摩尔/毫升之间（平均水平 = 2.13±0.37纳摩尔/毫升）。8名女性（53%）的MDA水平高于正常上限。3名患者的水平高于2.5纳摩尔/毫升，相当于第75百分位数。
本研究表明，测定血浆中MDA水平是研究癫痫女性脂质过氧化进而氧化应激的便捷方法。超过半数的癫痫女性（WWE）血浆中MDA水平较高，表明存在过量氧化应激。需要在更大队列中观察MDA水平与癫痫特征、抗癫痫药物治疗、营养状况及其他医疗状况之间的相关性。
