Kim Hye-Youn, Kim Yun-Ah, Lee Ho Sa, Rhie Ho Gun, Cho Shin-Hyeong, Yu Jae-Ran, Lee Sang-Eun
Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea.
Korean J Parasitol. 2009 Dec;47(4):413-5. doi: 10.3347/kjp.2009.47.4.413. Epub 2009 Dec 2.
Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5' and 3' ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.
在韩国对23份来自流浪猫的聚合酶链反应（PCR）阳性血液样本进行了弓形虫基因分型。我们使用了SAG2基因的2种不同的聚合酶链反应-限制性片段长度多态性（RFLP）模式，扩增该位点的5'端和3'端。结果显示，所有样本均属于I型克隆谱系。尽管未从样本中分离出弓形虫生物体，但本研究结果表明，感染弓形虫的流浪猫在我们的环境中应受到高度关注。需要制定充分且持续的流浪猫控制计划，以降低弓形虫作为一种威胁公众健康的人畜共患感染的传播风险。
