Mahami-Oskouei Mahmoud, Moradi Mohammad, Fallah Esmaeil, Hamidi Faezeh, Asl Rahnamaye Akbari Najibeh
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Dept. of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Parasitol. 2017 Jan-Mar;12(1):38-45.
, the obligate, intracellular parasite afflicts human in diverse ways such as ingestion of tissue cysts in undercooked meat. The aim of this study was to assess the prevalence and genotyping of isolated from meat samples being consumed in East Azerbaijan, Northwest of Iran.
Overall, 150 samples including chicken, beef and lamb meat were collected from retailers in different regions of Tabriz, northwestern Iran during April to September 2014. Molecular detection was done by amplifying B1 gene and surface antigen 2 (SAG2) loci. For genotyping of restriction fragment length polymorphism (RFLP) was performed using 3AI and I restriction enzymes. Finally, three positive isolate from each host was sequenced to evaluate and phylogenetic analysis.
Overall, 26 (17.33%) samples were positive for including 4 (8%) isolate from chicken, 8 (16%) isolates from cattle and 14 (28%) isolates from sheep. According to the RFLP patterns, sequencing and phylogenetic results, all of the samples were identified as genotype I.
The results indicated a potential risk of transmission of the disease through the consumption of infected meat. This is particularly important especially for pregnant women and immuno-compromised patients and this suggests that the training on the prevention of infection is necessary.
这种专性细胞内寄生虫以多种方式感染人类，例如食用未煮熟肉类中的组织囊肿。本研究的目的是评估从伊朗西北部东阿塞拜疆食用的肉类样本中分离出的[寄生虫名称未给出]的流行情况和基因分型。
2014年4月至9月期间，从伊朗西北部大不里士不同地区的零售商处共采集了150份样本，包括鸡肉、牛肉和羊肉。通过扩增B1基因和表面抗原2（SAG2）基因座进行分子检测。对于[寄生虫名称未给出]的基因分型，使用3AI和I限制性内切酶进行限制性片段长度多态性（RFLP）分析。最后，对每个宿主的三个阳性分离株进行测序以进行评估和系统发育分析。
总体而言，26份（17.33%）样本的[寄生虫名称未给出]呈阳性，其中包括4份（8%）鸡肉分离株、8份（16%）牛分离株和14份（28%）羊分离株。根据RFLP模式、测序和系统发育结果，所有样本均被鉴定为I型基因型。
结果表明通过食用受感染肉类存在疾病传播的潜在风险。这对于孕妇和免疫功能低下的患者尤为重要，这表明有必要开展预防感染的培训。
