Razmi G R, Maleki M, Farzaneh N, Talebkhan Garoussi M, Fallah A H
Department of Pathobiology, School Veterinary Medicine, Ferdowsi University of Mashhad, P.O. Box 91775-1793, Mashhad, Iran.
Parasitol Res. 2007 Mar;100(4):755-7. doi: 10.1007/s00436-006-0325-6. Epub 2006 Oct 6.
Neospora caninum is an apicomplexan protozoan which causes abortion in cattle worldwide. The present study was designed to assess the importance of bovine neosporosis for causing abortion in Iranian cattle. Infection was primarily diagnosed by polymerase chain reaction (PCR), complemented with histopathology and immunohistochemistry (IHC). One hundred brains of aborted bovine fetuses were collected from Iranian dairy herd between 2003 and 2005 in Mashhad area. N. caninum was detected by PCR in the brains of 13 aborted fetuses. Lesions consistent with N. caninum infection were observed in 12 of fetal brains. Immunohistochemical examination of brain detected N. caninum organisms in three aborted fetuses, and a thick-walled (2 mum) cyst with 50 mum diameter was identified in one of the IHC-positive brain. The results indicated that neosporosis is an important cause of abortion in dairy cattle of Iran.
犬新孢子虫是一种顶复门原生动物，可导致全球范围内的牛流产。本研究旨在评估牛新孢子虫病在伊朗牛流产中的重要性。感染主要通过聚合酶链反应（PCR）诊断，并辅以组织病理学和免疫组织化学（IHC）。2003年至2005年间，从马什哈德地区的伊朗奶牛群中收集了100个流产牛胎儿的大脑。通过PCR在13个流产胎儿的大脑中检测到犬新孢子虫。在12个胎儿大脑中观察到与犬新孢子虫感染一致的病变。对大脑进行免疫组织化学检查，在3个流产胎儿中检测到犬新孢子虫生物体，在其中一个免疫组织化学阳性的大脑中鉴定出一个直径50μm、壁厚2μm的囊肿。结果表明，新孢子虫病是伊朗奶牛流产的一个重要原因。
